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Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156

Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To investigate the molecular mechanisms underlying this effect, maize endosperms were collected from Zea ma...

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Autores principales: Huang, Huanhuan, Xie, Sidi, Xiao, Qianlin, Wei, Bin, Zheng, Lanjie, Wang, Yongbin, Cao, Yao, Zhang, Xiangge, Long, Tiandan, Li, Yangping, Hu, Yufeng, Yu, Guowu, Liu, Hanmei, Liu, Yinghong, Huang, Zhi, Zhang, Junjie, Huang, Yubi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901336/
https://www.ncbi.nlm.nih.gov/pubmed/27282997
http://dx.doi.org/10.1038/srep27590
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author Huang, Huanhuan
Xie, Sidi
Xiao, Qianlin
Wei, Bin
Zheng, Lanjie
Wang, Yongbin
Cao, Yao
Zhang, Xiangge
Long, Tiandan
Li, Yangping
Hu, Yufeng
Yu, Guowu
Liu, Hanmei
Liu, Yinghong
Huang, Zhi
Zhang, Junjie
Huang, Yubi
author_facet Huang, Huanhuan
Xie, Sidi
Xiao, Qianlin
Wei, Bin
Zheng, Lanjie
Wang, Yongbin
Cao, Yao
Zhang, Xiangge
Long, Tiandan
Li, Yangping
Hu, Yufeng
Yu, Guowu
Liu, Hanmei
Liu, Yinghong
Huang, Zhi
Zhang, Junjie
Huang, Yubi
author_sort Huang, Huanhuan
collection PubMed
description Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To investigate the molecular mechanisms underlying this effect, maize endosperms were collected from Zea mays L. B73 inbred line 10 d after pollination and treated with sucrose, ABA, or sucrose plus ABA at 28 °C in the dark for 24 h. RNA-sequence analysis of the maize endosperm transcriptome revealed 47 candidate transcription factors among the differentially expressed genes. We therefore speculate that starch synthetic gene expression is regulated by transcription factors induced by the combination of sucrose and ABA. ZmEREB156, a candidate transcription factor, is induced by sucrose plus ABA and is involved in starch biosynthesis. The ZmEREB156-GFP-fused protein was localized in the nuclei of onion epidermal cells, and ZmEREB156 protein possessed strong transcriptional activation activity. Promoter activity of the starch-related genes Zmsh2 and ZmSSIIIa increased after overexpression of ZmEREB156 in maize endosperm. ZmEREB156 could bind to the ZmSSIIIa promoter but not the Zmsh2 promoter in a yeast one-hybrid system. Thus, ZmEREB156 positively modulates starch biosynthetic gene ZmSSIIIa via the synergistic effect of sucrose and ABA.
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spelling pubmed-49013362016-06-13 Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156 Huang, Huanhuan Xie, Sidi Xiao, Qianlin Wei, Bin Zheng, Lanjie Wang, Yongbin Cao, Yao Zhang, Xiangge Long, Tiandan Li, Yangping Hu, Yufeng Yu, Guowu Liu, Hanmei Liu, Yinghong Huang, Zhi Zhang, Junjie Huang, Yubi Sci Rep Article Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To investigate the molecular mechanisms underlying this effect, maize endosperms were collected from Zea mays L. B73 inbred line 10 d after pollination and treated with sucrose, ABA, or sucrose plus ABA at 28 °C in the dark for 24 h. RNA-sequence analysis of the maize endosperm transcriptome revealed 47 candidate transcription factors among the differentially expressed genes. We therefore speculate that starch synthetic gene expression is regulated by transcription factors induced by the combination of sucrose and ABA. ZmEREB156, a candidate transcription factor, is induced by sucrose plus ABA and is involved in starch biosynthesis. The ZmEREB156-GFP-fused protein was localized in the nuclei of onion epidermal cells, and ZmEREB156 protein possessed strong transcriptional activation activity. Promoter activity of the starch-related genes Zmsh2 and ZmSSIIIa increased after overexpression of ZmEREB156 in maize endosperm. ZmEREB156 could bind to the ZmSSIIIa promoter but not the Zmsh2 promoter in a yeast one-hybrid system. Thus, ZmEREB156 positively modulates starch biosynthetic gene ZmSSIIIa via the synergistic effect of sucrose and ABA. Nature Publishing Group 2016-06-10 /pmc/articles/PMC4901336/ /pubmed/27282997 http://dx.doi.org/10.1038/srep27590 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Huang, Huanhuan
Xie, Sidi
Xiao, Qianlin
Wei, Bin
Zheng, Lanjie
Wang, Yongbin
Cao, Yao
Zhang, Xiangge
Long, Tiandan
Li, Yangping
Hu, Yufeng
Yu, Guowu
Liu, Hanmei
Liu, Yinghong
Huang, Zhi
Zhang, Junjie
Huang, Yubi
Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156
title Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156
title_full Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156
title_fullStr Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156
title_full_unstemmed Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156
title_short Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156
title_sort sucrose and aba regulate starch biosynthesis in maize through a novel transcription factor, zmereb156
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901336/
https://www.ncbi.nlm.nih.gov/pubmed/27282997
http://dx.doi.org/10.1038/srep27590
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