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Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro

BACKGROUND: Corneal tensile strain increases if the cornea becomes thin or if intraocular pressure increases. However, the effects of mechanical stress on extracellular matrix (ECM) remodelling in the corneal repair process and the corneal anomalies are unknown. METHODS: In this study, the combined...

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Autores principales: Feng, Pengfei, Li, Xiaona, Chen, Weiyi, Liu, Chengxing, Rong, Shuo, Wang, Xiaojun, Du, Genlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901398/
https://www.ncbi.nlm.nih.gov/pubmed/27286674
http://dx.doi.org/10.1186/s12938-016-0198-6
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author Feng, Pengfei
Li, Xiaona
Chen, Weiyi
Liu, Chengxing
Rong, Shuo
Wang, Xiaojun
Du, Genlai
author_facet Feng, Pengfei
Li, Xiaona
Chen, Weiyi
Liu, Chengxing
Rong, Shuo
Wang, Xiaojun
Du, Genlai
author_sort Feng, Pengfei
collection PubMed
description BACKGROUND: Corneal tensile strain increases if the cornea becomes thin or if intraocular pressure increases. However, the effects of mechanical stress on extracellular matrix (ECM) remodelling in the corneal repair process and the corneal anomalies are unknown. METHODS: In this study, the combined effects of interleukin-1β (IL-1β) on matrix metalloproteinases (MMPs) in corneal fibroblasts under cyclic stretching were investigated in vitro. Cultured rabbit corneal fibroblasts were subjected to 5, 10 or 15 % cyclic equibiaxial stretching at 0.1 Hz for 36 h in the presence of IL-1β. Conditioned medium was harvested for the analysis of MMP2 and MMP9 protein production using the gelatin zymography and western blot techniques. RESULTS AND CONCLUSIONS: Cyclic equibiaxial stretching changed the cell morphology by increasing the contractility of F-actin fibres. IL-1β alone induced the expression of MMP9 and increased the production of MMP2, and 5 % stretching alone decreased the production of MMP2, which indicates that a low stretching magnitude can reduce ECM degradation. In the presence of IL-1β, 5 and 10 % stretching increased the production of MMP2, whereas 15 % stretching increased the production of MMP9. These results indicate that MMP expression is enhanced by cyclic mechanical stimulation in the presence of IL-1β, which is expected to contribute to corneal ECM degradation, leading to the development of post-refractive surgery keratectasia.
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spelling pubmed-49013982016-06-11 Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro Feng, Pengfei Li, Xiaona Chen, Weiyi Liu, Chengxing Rong, Shuo Wang, Xiaojun Du, Genlai Biomed Eng Online Research BACKGROUND: Corneal tensile strain increases if the cornea becomes thin or if intraocular pressure increases. However, the effects of mechanical stress on extracellular matrix (ECM) remodelling in the corneal repair process and the corneal anomalies are unknown. METHODS: In this study, the combined effects of interleukin-1β (IL-1β) on matrix metalloproteinases (MMPs) in corneal fibroblasts under cyclic stretching were investigated in vitro. Cultured rabbit corneal fibroblasts were subjected to 5, 10 or 15 % cyclic equibiaxial stretching at 0.1 Hz for 36 h in the presence of IL-1β. Conditioned medium was harvested for the analysis of MMP2 and MMP9 protein production using the gelatin zymography and western blot techniques. RESULTS AND CONCLUSIONS: Cyclic equibiaxial stretching changed the cell morphology by increasing the contractility of F-actin fibres. IL-1β alone induced the expression of MMP9 and increased the production of MMP2, and 5 % stretching alone decreased the production of MMP2, which indicates that a low stretching magnitude can reduce ECM degradation. In the presence of IL-1β, 5 and 10 % stretching increased the production of MMP2, whereas 15 % stretching increased the production of MMP9. These results indicate that MMP expression is enhanced by cyclic mechanical stimulation in the presence of IL-1β, which is expected to contribute to corneal ECM degradation, leading to the development of post-refractive surgery keratectasia. BioMed Central 2016-06-10 /pmc/articles/PMC4901398/ /pubmed/27286674 http://dx.doi.org/10.1186/s12938-016-0198-6 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Feng, Pengfei
Li, Xiaona
Chen, Weiyi
Liu, Chengxing
Rong, Shuo
Wang, Xiaojun
Du, Genlai
Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
title Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
title_full Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
title_fullStr Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
title_full_unstemmed Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
title_short Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
title_sort combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901398/
https://www.ncbi.nlm.nih.gov/pubmed/27286674
http://dx.doi.org/10.1186/s12938-016-0198-6
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