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Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis

OBJECTIVES: Despite clinical evidence demonstrating that meniscus tears are early events in the initiation and propagation of knee osteoarthritis (OA), the biology of how meniscus injury leads to changes in the articular cartilage is not well studied. Therefore, we compared the molecular biology of...

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Autores principales: Brophy, Robert H., Sandell, Linda, Wright, Rick W., Rai, Muhammad Farooq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901622/
http://dx.doi.org/10.1177/2325967115S00065
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author Brophy, Robert H.
Sandell, Linda
Wright, Rick W.
Rai, Muhammad Farooq
author_facet Brophy, Robert H.
Sandell, Linda
Wright, Rick W.
Rai, Muhammad Farooq
author_sort Brophy, Robert H.
collection PubMed
description OBJECTIVES: Despite clinical evidence demonstrating that meniscus tears are early events in the initiation and propagation of knee osteoarthritis (OA), the biology of how meniscus injury leads to changes in the articular cartilage is not well studied. Therefore, we compared the molecular biology of articular and meniscal cartilage within the same knees undergoing partial meniscectomy to advance our understanding of early molecular events in the knee that contribute to the development of OA following a meniscus tear. The purpose of this study was to test the hypothesis that meniscal and articular cartilage from knees undergoing arthroscopic partial meniscectomy will demonstrate molecular signature representative of early OA. METHODS: Patients (n=12) with a known isolated medial meniscus tear without any knee chondrosis or radiographic OA were consented prior to surgery. During arthroscopic partial meniscectomy, a sample of their injured meniscus and a small fragment of their articular cartilage off the medial femoral condyle were procured. The transcriptome signatures measured through Affymetrix microarray were compared between the two tissues and underlying biological processes were explored computationally. RESULTS: 3566 gene transcripts, representing several underlying biological processes, including extracellular matrix organization, wound healing, cell adhesion, chemotaxis, blood vessel morphogenesis, and angiogenesis, were differentially expressed between meniscus and articular cartilage. CONCLUSION: Articular cartilage and meniscus exhibit molecular events associated with OA at the time of partial meniscectomy despite no gross evidence of degeneration in the knee. These findings identify early effects of meniscus injury on meniscus and articular cartilage, which likely lead to the development of knee OA.
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spelling pubmed-49016222016-06-10 Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis Brophy, Robert H. Sandell, Linda Wright, Rick W. Rai, Muhammad Farooq Orthop J Sports Med Article OBJECTIVES: Despite clinical evidence demonstrating that meniscus tears are early events in the initiation and propagation of knee osteoarthritis (OA), the biology of how meniscus injury leads to changes in the articular cartilage is not well studied. Therefore, we compared the molecular biology of articular and meniscal cartilage within the same knees undergoing partial meniscectomy to advance our understanding of early molecular events in the knee that contribute to the development of OA following a meniscus tear. The purpose of this study was to test the hypothesis that meniscal and articular cartilage from knees undergoing arthroscopic partial meniscectomy will demonstrate molecular signature representative of early OA. METHODS: Patients (n=12) with a known isolated medial meniscus tear without any knee chondrosis or radiographic OA were consented prior to surgery. During arthroscopic partial meniscectomy, a sample of their injured meniscus and a small fragment of their articular cartilage off the medial femoral condyle were procured. The transcriptome signatures measured through Affymetrix microarray were compared between the two tissues and underlying biological processes were explored computationally. RESULTS: 3566 gene transcripts, representing several underlying biological processes, including extracellular matrix organization, wound healing, cell adhesion, chemotaxis, blood vessel morphogenesis, and angiogenesis, were differentially expressed between meniscus and articular cartilage. CONCLUSION: Articular cartilage and meniscus exhibit molecular events associated with OA at the time of partial meniscectomy despite no gross evidence of degeneration in the knee. These findings identify early effects of meniscus injury on meniscus and articular cartilage, which likely lead to the development of knee OA. SAGE Publications 2015-07-17 /pmc/articles/PMC4901622/ http://dx.doi.org/10.1177/2325967115S00065 Text en © The Author(s) 2015 http://creativecommons.org/licenses/by-nc-nd/3.0/ This open-access article is published and distributed under the Creative Commons Attribution - NonCommercial - No Derivatives License (http://creativecommons.org/licenses/by-nc-nd/3.0/), which permits the noncommercial use, distribution, and reproduction of the article in any medium, provided the original author and source are credited. You may not alter, transform, or build upon this article without the permission of the Author(s). For reprints and permission queries, please visit SAGE’s Web site at http://www.sagepub.com/journalsPermissions.nav.
spellingShingle Article
Brophy, Robert H.
Sandell, Linda
Wright, Rick W.
Rai, Muhammad Farooq
Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis
title Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis
title_full Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis
title_fullStr Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis
title_full_unstemmed Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis
title_short Transcriptomic Signatures of Meniscal Tears and Articular Cartilage from Knees Undergoing Arthroscopic Partial Meniscectomy Show Evidence for Early Osteoarthritis
title_sort transcriptomic signatures of meniscal tears and articular cartilage from knees undergoing arthroscopic partial meniscectomy show evidence for early osteoarthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901622/
http://dx.doi.org/10.1177/2325967115S00065
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