Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur

Short phosphorothioate oligonucleotides have been prepared by refluxing an equimolar mixture of thymidine and triethylammonium phosphite in toluene in the presence of elemental sulfur. Desulfurization and subsequent digestion of the products by P1 nuclease revealed that nearly 80% of the internucleo...

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Detalles Bibliográficos
Autor principal: Lönnberg, Tuomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2016
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902050/
https://www.ncbi.nlm.nih.gov/pubmed/27340459
http://dx.doi.org/10.3762/bjoc.12.67
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author Lönnberg, Tuomas
author_facet Lönnberg, Tuomas
author_sort Lönnberg, Tuomas
collection PubMed
description Short phosphorothioate oligonucleotides have been prepared by refluxing an equimolar mixture of thymidine and triethylammonium phosphite in toluene in the presence of elemental sulfur. Desulfurization and subsequent digestion of the products by P1 nuclease revealed that nearly 80% of the internucleosidic linkages thus formed were of the canonical 3´,5´-type.
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spelling pubmed-49020502016-06-23 Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur Lönnberg, Tuomas Beilstein J Org Chem Full Research Paper Short phosphorothioate oligonucleotides have been prepared by refluxing an equimolar mixture of thymidine and triethylammonium phosphite in toluene in the presence of elemental sulfur. Desulfurization and subsequent digestion of the products by P1 nuclease revealed that nearly 80% of the internucleosidic linkages thus formed were of the canonical 3´,5´-type. Beilstein-Institut 2016-04-11 /pmc/articles/PMC4902050/ /pubmed/27340459 http://dx.doi.org/10.3762/bjoc.12.67 Text en Copyright © 2016, Lönnberg https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Full Research Paper
Lönnberg, Tuomas
Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
title Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
title_full Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
title_fullStr Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
title_full_unstemmed Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
title_short Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
title_sort nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902050/
https://www.ncbi.nlm.nih.gov/pubmed/27340459
http://dx.doi.org/10.3762/bjoc.12.67
work_keys_str_mv AT lonnbergtuomas nucleicacidsthroughcondensationofnucleosidesandphosphorousacidinthepresenceofsulfur

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