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DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences
This paper describes the design, construction and characterization of two DNA molecules that function as prototype Standard Reference Materials for use in the determination of DNA sequences. The first prototype reference DNA molecule was constructed to function as a true reference DNA sequence encod...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
[Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902566/ https://www.ncbi.nlm.nih.gov/pubmed/27805128 http://dx.doi.org/10.6028/jres.102.005 |
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author | McKenney, Keith Hoskins, Joel Tian, Jingxiang Reddy, Prasad |
author_facet | McKenney, Keith Hoskins, Joel Tian, Jingxiang Reddy, Prasad |
author_sort | McKenney, Keith |
collection | PubMed |
description | This paper describes the design, construction and characterization of two DNA molecules that function as prototype Standard Reference Materials for use in the determination of DNA sequences. The first prototype reference DNA molecule was constructed to function as a true reference DNA sequence encoding the human mitochondrial hypervariable region 1. The second DNA molecule was designed to be a molecular DNA “tag” for use in the identification of DNA Standard Reference Materials. DNA molecules were chemically and enzymatically synthesized, cloned in molecular cloning vectors and sequenced using manual and automated fluorescent DNA sequencing methods. The sequence of the cloned human mitochondrial HV1 DNA shows six base pair differences from the original published DNA sequence. The molecular DNA tag sequence uses the universal genetic code with one letter amino acid abbreviations to spell the words “NIST DNA STANDARD REFERENCE MATERIAL.” The need and utility of an expanded molecular code to uniquely identify DNA molecules is discussed. The use of these DNA molecules in the development of DNA Standard Reference Materials is also described. |
format | Online Article Text |
id | pubmed-4902566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49025662016-10-28 DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences McKenney, Keith Hoskins, Joel Tian, Jingxiang Reddy, Prasad J Res Natl Inst Stand Technol Article This paper describes the design, construction and characterization of two DNA molecules that function as prototype Standard Reference Materials for use in the determination of DNA sequences. The first prototype reference DNA molecule was constructed to function as a true reference DNA sequence encoding the human mitochondrial hypervariable region 1. The second DNA molecule was designed to be a molecular DNA “tag” for use in the identification of DNA Standard Reference Materials. DNA molecules were chemically and enzymatically synthesized, cloned in molecular cloning vectors and sequenced using manual and automated fluorescent DNA sequencing methods. The sequence of the cloned human mitochondrial HV1 DNA shows six base pair differences from the original published DNA sequence. The molecular DNA tag sequence uses the universal genetic code with one letter amino acid abbreviations to spell the words “NIST DNA STANDARD REFERENCE MATERIAL.” The need and utility of an expanded molecular code to uniquely identify DNA molecules is discussed. The use of these DNA molecules in the development of DNA Standard Reference Materials is also described. [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 1997 /pmc/articles/PMC4902566/ /pubmed/27805128 http://dx.doi.org/10.6028/jres.102.005 Text en https://creativecommons.org/publicdomain/zero/1.0/ The Journal of Research of the National Institute of Standards and Technology is a publication of the U.S. Government. The papers are in the public domain and are not subject to copyright in the United States. Articles from J Res may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright. |
spellingShingle | Article McKenney, Keith Hoskins, Joel Tian, Jingxiang Reddy, Prasad DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences |
title | DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences |
title_full | DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences |
title_fullStr | DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences |
title_full_unstemmed | DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences |
title_short | DNA Molecules as Standard Reference Materials I: Development of DNA Identification Sequences and Human Mitochondrial DNA Reference Sequences |
title_sort | dna molecules as standard reference materials i: development of dna identification sequences and human mitochondrial dna reference sequences |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902566/ https://www.ncbi.nlm.nih.gov/pubmed/27805128 http://dx.doi.org/10.6028/jres.102.005 |
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