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Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells

BACKGROUND: Endothelin-1 (ET-1) is involved in pulmonary vascular remodeling. The aim of this study was to investigate the biochemical interactions between PPAR-γ, TGF-β1 and ET-1 in vitro. METHODS: A549 cells were pre-treated with S2505 (10 μM), S2871 (10 μM) with/without SB203580 (10 μM) for 60 mi...

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Autores principales: Xiang, Shulin, Zeng, Yi, Xiong, Bin, Qin, Yueqiu, Huang, Xia, Jiang, Yujie, Luo, Weigui, Sooranna, Suren R., Pinhu, Liao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902962/
https://www.ncbi.nlm.nih.gov/pubmed/27293383
http://dx.doi.org/10.1186/s12950-016-0128-1
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author Xiang, Shulin
Zeng, Yi
Xiong, Bin
Qin, Yueqiu
Huang, Xia
Jiang, Yujie
Luo, Weigui
Sooranna, Suren R.
Pinhu, Liao
author_facet Xiang, Shulin
Zeng, Yi
Xiong, Bin
Qin, Yueqiu
Huang, Xia
Jiang, Yujie
Luo, Weigui
Sooranna, Suren R.
Pinhu, Liao
author_sort Xiang, Shulin
collection PubMed
description BACKGROUND: Endothelin-1 (ET-1) is involved in pulmonary vascular remodeling. The aim of this study was to investigate the biochemical interactions between PPAR-γ, TGF-β1 and ET-1 in vitro. METHODS: A549 cells were pre-treated with S2505 (10 μM), S2871 (10 μM) with/without SB203580 (10 μM) for 60 min following 2 h treatment with 10 ng/mL TGF-β1. A549 cells were also transfected with positive or negative PPAR-γ plasmids for comparison. RT-PCR, ELISA, western blotting and confocal laser scanning microscopy (CLSM) were used to measure the relevant expression of mRNA, protein, mediators of pathways and nuclear factor translocation. RESULTS: SB203580 inhibited TGF-β1 induced ET-1 expression in A549 cells. S2871 decreased PPAR-γ mRNA and increase TGF-β1-induced ET-1 expression. S2871 increased phosphorylation of p38 MAPK and Smad2. Cells transfected with PPAR-γ negative plasmid increased TGF-β1 induced ET-1 expression, and increased the expression of phospho-p38 MAPK and phospho-Smad2. S2505 increased PPAR-γ mRNA expression, suppressed the increased TGF-β1-induced expression of ET-1. S2505 inhibited TGF-β1 induced phosphorylation of p38 MAPK and Smad2, also the nuclear translocation of Smad2. Cells transfected with PPAR-γ positive plasmid reduced TGF-β1-induced ET-1 expression, and inhibited the expression of phospho-p38 MAPK and phospho-Smad2. CONCLUSIONS: TGF-β1 induced release of endothelin-1 is PPAR-γ dependent in cultured A549 cells.
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spelling pubmed-49029622016-06-12 Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells Xiang, Shulin Zeng, Yi Xiong, Bin Qin, Yueqiu Huang, Xia Jiang, Yujie Luo, Weigui Sooranna, Suren R. Pinhu, Liao J Inflamm (Lond) Research BACKGROUND: Endothelin-1 (ET-1) is involved in pulmonary vascular remodeling. The aim of this study was to investigate the biochemical interactions between PPAR-γ, TGF-β1 and ET-1 in vitro. METHODS: A549 cells were pre-treated with S2505 (10 μM), S2871 (10 μM) with/without SB203580 (10 μM) for 60 min following 2 h treatment with 10 ng/mL TGF-β1. A549 cells were also transfected with positive or negative PPAR-γ plasmids for comparison. RT-PCR, ELISA, western blotting and confocal laser scanning microscopy (CLSM) were used to measure the relevant expression of mRNA, protein, mediators of pathways and nuclear factor translocation. RESULTS: SB203580 inhibited TGF-β1 induced ET-1 expression in A549 cells. S2871 decreased PPAR-γ mRNA and increase TGF-β1-induced ET-1 expression. S2871 increased phosphorylation of p38 MAPK and Smad2. Cells transfected with PPAR-γ negative plasmid increased TGF-β1 induced ET-1 expression, and increased the expression of phospho-p38 MAPK and phospho-Smad2. S2505 increased PPAR-γ mRNA expression, suppressed the increased TGF-β1-induced expression of ET-1. S2505 inhibited TGF-β1 induced phosphorylation of p38 MAPK and Smad2, also the nuclear translocation of Smad2. Cells transfected with PPAR-γ positive plasmid reduced TGF-β1-induced ET-1 expression, and inhibited the expression of phospho-p38 MAPK and phospho-Smad2. CONCLUSIONS: TGF-β1 induced release of endothelin-1 is PPAR-γ dependent in cultured A549 cells. BioMed Central 2016-06-10 /pmc/articles/PMC4902962/ /pubmed/27293383 http://dx.doi.org/10.1186/s12950-016-0128-1 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xiang, Shulin
Zeng, Yi
Xiong, Bin
Qin, Yueqiu
Huang, Xia
Jiang, Yujie
Luo, Weigui
Sooranna, Suren R.
Pinhu, Liao
Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells
title Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells
title_full Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells
title_fullStr Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells
title_full_unstemmed Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells
title_short Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells
title_sort transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in a549 cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902962/
https://www.ncbi.nlm.nih.gov/pubmed/27293383
http://dx.doi.org/10.1186/s12950-016-0128-1
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