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Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source

Food-borne campylobacteriosis is caused mainly by the handling or consumption of undercooked chicken meat or by the ingestion of contaminated raw milk. Knowledge about the contributions of different food sources to gastrointestinal disease is fundamental to prioritize food safety interventions and t...

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Autores principales: Di Giannatale, Elisabetta, Garofolo, Giuliano, Alessiani, Alessandra, Di Donato, Guido, Candeloro, Luca, Vencia, Walter, Decastelli, Lucia, Marotta, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904018/
https://www.ncbi.nlm.nih.gov/pubmed/27379033
http://dx.doi.org/10.3389/fmicb.2016.00887
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author Di Giannatale, Elisabetta
Garofolo, Giuliano
Alessiani, Alessandra
Di Donato, Guido
Candeloro, Luca
Vencia, Walter
Decastelli, Lucia
Marotta, Francesca
author_facet Di Giannatale, Elisabetta
Garofolo, Giuliano
Alessiani, Alessandra
Di Donato, Guido
Candeloro, Luca
Vencia, Walter
Decastelli, Lucia
Marotta, Francesca
author_sort Di Giannatale, Elisabetta
collection PubMed
description Food-borne campylobacteriosis is caused mainly by the handling or consumption of undercooked chicken meat or by the ingestion of contaminated raw milk. Knowledge about the contributions of different food sources to gastrointestinal disease is fundamental to prioritize food safety interventions and to establish proper control strategies. Assessing the genetic diversity among Campylobacter species is essential to our understanding of their epidemiology and population structure. We molecularly characterized 56 Campylobacter jejuni isolates (31 from patients hospitalized with gastroenteritis, 17 from raw milk samples, and 8 from chicken samples) using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) in order to trace the source of the disease. We also used a population genetic approach to investigate the source of the human cases from six different reservoirs of infection. MLST identified 25 different sequence types and 11 clonal complexes (CCs) (21, 658, 206, 353, 443, 48, 61, 257, 1332, 354, 574) and these included several alleles not cited previously in the PubMLST international database. The most prevalent CCs were 21, 206, and 354. PFGE showed 34 pulsotypes divided between 28 different clusters. At the fine scale, by means of PFGE and MLST, only two human cases were linked to raw milk, while one case was linked to chicken meat. The investigation revealed the presence of several genotypes among the human isolates, which probably suggests multiple foci for the infections. Finally, the source attribution model we used revealed that most cases were attributed to chicken (69.75%) as the main reservoir in Italy, followed to a lesser extent by the following sources: cattle (8.25%); environment (6.28%); wild bird (7.37%); small ruminant (5.35%), and pork (2.98%). This study confirms the importance of correlating epidemiological investigations with molecular epidemiological data to better understand the dynamics of infection.
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spelling pubmed-49040182016-07-04 Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source Di Giannatale, Elisabetta Garofolo, Giuliano Alessiani, Alessandra Di Donato, Guido Candeloro, Luca Vencia, Walter Decastelli, Lucia Marotta, Francesca Front Microbiol Microbiology Food-borne campylobacteriosis is caused mainly by the handling or consumption of undercooked chicken meat or by the ingestion of contaminated raw milk. Knowledge about the contributions of different food sources to gastrointestinal disease is fundamental to prioritize food safety interventions and to establish proper control strategies. Assessing the genetic diversity among Campylobacter species is essential to our understanding of their epidemiology and population structure. We molecularly characterized 56 Campylobacter jejuni isolates (31 from patients hospitalized with gastroenteritis, 17 from raw milk samples, and 8 from chicken samples) using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) in order to trace the source of the disease. We also used a population genetic approach to investigate the source of the human cases from six different reservoirs of infection. MLST identified 25 different sequence types and 11 clonal complexes (CCs) (21, 658, 206, 353, 443, 48, 61, 257, 1332, 354, 574) and these included several alleles not cited previously in the PubMLST international database. The most prevalent CCs were 21, 206, and 354. PFGE showed 34 pulsotypes divided between 28 different clusters. At the fine scale, by means of PFGE and MLST, only two human cases were linked to raw milk, while one case was linked to chicken meat. The investigation revealed the presence of several genotypes among the human isolates, which probably suggests multiple foci for the infections. Finally, the source attribution model we used revealed that most cases were attributed to chicken (69.75%) as the main reservoir in Italy, followed to a lesser extent by the following sources: cattle (8.25%); environment (6.28%); wild bird (7.37%); small ruminant (5.35%), and pork (2.98%). This study confirms the importance of correlating epidemiological investigations with molecular epidemiological data to better understand the dynamics of infection. Frontiers Media S.A. 2016-06-13 /pmc/articles/PMC4904018/ /pubmed/27379033 http://dx.doi.org/10.3389/fmicb.2016.00887 Text en Copyright © 2016 Di Giannatale, Garofolo, Alessiani, Di Donato, Candeloro, Vencia, Decastelli and Marotta. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Di Giannatale, Elisabetta
Garofolo, Giuliano
Alessiani, Alessandra
Di Donato, Guido
Candeloro, Luca
Vencia, Walter
Decastelli, Lucia
Marotta, Francesca
Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source
title Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source
title_full Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source
title_fullStr Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source
title_full_unstemmed Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source
title_short Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source
title_sort tracing back clinical campylobacter jejuni in the northwest of italy and assessing their potential source
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904018/
https://www.ncbi.nlm.nih.gov/pubmed/27379033
http://dx.doi.org/10.3389/fmicb.2016.00887
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