Cargando…
Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species
Several Artemisia species are used as herbal medicines including the dried aerial parts of Artemisia capillaris, which are used as Artemisiae Capillaris Herba (known as “Injinho” in Korean medicinal terminology and “Yin Chen Hao” in Chinese). In this study, we developed tools for distinguishing betw...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904105/ https://www.ncbi.nlm.nih.gov/pubmed/27313651 http://dx.doi.org/10.1155/2016/7043436 |
_version_ | 1782437096687927296 |
---|---|
author | Doh, Eui Jeong Paek, Seung-Ho Lee, Guemsan Lee, Mi-Young Oh, Seung-Eun |
author_facet | Doh, Eui Jeong Paek, Seung-Ho Lee, Guemsan Lee, Mi-Young Oh, Seung-Eun |
author_sort | Doh, Eui Jeong |
collection | PubMed |
description | Several Artemisia species are used as herbal medicines including the dried aerial parts of Artemisia capillaris, which are used as Artemisiae Capillaris Herba (known as “Injinho” in Korean medicinal terminology and “Yin Chen Hao” in Chinese). In this study, we developed tools for distinguishing between A. capillaris and 11 other Artemisia species that grow and/or are cultured in China, Japan, and Korea. Based on partial nucleotide sequences in the internal transcribed spacer (ITS) that differ between the species, we designed primers to amplify a DNA marker for A. capillaris. In addition, to detect other Artemisia species that are contaminants of A. capillaris, we designed primers to amplify DNA markers of A. japonica, A. annua, A. apiacea, and A. anomala. Moreover, based on random amplified polymorphic DNA analysis, we confirmed that primers developed in a previous study could be used to identify Artemisia species that are sources of Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba. By using these primers, we found that multiplex polymerase chain reaction (PCR) was a reliable tool to distinguish between A. capillaris and other Artemisia species and to identify other Artemisia species as contaminants of A. capillaris in a single PCR. |
format | Online Article Text |
id | pubmed-4904105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49041052016-06-16 Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species Doh, Eui Jeong Paek, Seung-Ho Lee, Guemsan Lee, Mi-Young Oh, Seung-Eun Evid Based Complement Alternat Med Research Article Several Artemisia species are used as herbal medicines including the dried aerial parts of Artemisia capillaris, which are used as Artemisiae Capillaris Herba (known as “Injinho” in Korean medicinal terminology and “Yin Chen Hao” in Chinese). In this study, we developed tools for distinguishing between A. capillaris and 11 other Artemisia species that grow and/or are cultured in China, Japan, and Korea. Based on partial nucleotide sequences in the internal transcribed spacer (ITS) that differ between the species, we designed primers to amplify a DNA marker for A. capillaris. In addition, to detect other Artemisia species that are contaminants of A. capillaris, we designed primers to amplify DNA markers of A. japonica, A. annua, A. apiacea, and A. anomala. Moreover, based on random amplified polymorphic DNA analysis, we confirmed that primers developed in a previous study could be used to identify Artemisia species that are sources of Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba. By using these primers, we found that multiplex polymerase chain reaction (PCR) was a reliable tool to distinguish between A. capillaris and other Artemisia species and to identify other Artemisia species as contaminants of A. capillaris in a single PCR. Hindawi Publishing Corporation 2016 2016-05-30 /pmc/articles/PMC4904105/ /pubmed/27313651 http://dx.doi.org/10.1155/2016/7043436 Text en Copyright © 2016 Eui Jeong Doh et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Doh, Eui Jeong Paek, Seung-Ho Lee, Guemsan Lee, Mi-Young Oh, Seung-Eun Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species |
title | Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species |
title_full | Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species |
title_fullStr | Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species |
title_full_unstemmed | Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species |
title_short | Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species |
title_sort | application of partial internal transcribed spacer sequences for the discrimination of artemisia capillaris from other artemisia species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904105/ https://www.ncbi.nlm.nih.gov/pubmed/27313651 http://dx.doi.org/10.1155/2016/7043436 |
work_keys_str_mv | AT doheuijeong applicationofpartialinternaltranscribedspacersequencesforthediscriminationofartemisiacapillarisfromotherartemisiaspecies AT paekseungho applicationofpartialinternaltranscribedspacersequencesforthediscriminationofartemisiacapillarisfromotherartemisiaspecies AT leeguemsan applicationofpartialinternaltranscribedspacersequencesforthediscriminationofartemisiacapillarisfromotherartemisiaspecies AT leemiyoung applicationofpartialinternaltranscribedspacersequencesforthediscriminationofartemisiacapillarisfromotherartemisiaspecies AT ohseungeun applicationofpartialinternaltranscribedspacersequencesforthediscriminationofartemisiacapillarisfromotherartemisiaspecies |