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Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway

Chronic exposures to arsenic had been associated with metabolism diseases. Peroxisome proliferator-activated receptor gamma (PPARγ) was found in the liver, regulated metabolism. Here, we found that the expression of PPARγ was decreased, the generation of reactive oxygen species (ROS) and autophagy w...

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Autores principales: Bai, Jie, Yao, Xiaofeng, Jiang, Liping, Zhang, Qiaoting, Guan, Huai, Liu, Shuang, Wu, Wei, Qiu, Tianming, Gao, Ni, Yang, Lei, Yang, Guang, Sun, Xiance
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904213/
https://www.ncbi.nlm.nih.gov/pubmed/27291853
http://dx.doi.org/10.1038/srep27733
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author Bai, Jie
Yao, Xiaofeng
Jiang, Liping
Zhang, Qiaoting
Guan, Huai
Liu, Shuang
Wu, Wei
Qiu, Tianming
Gao, Ni
Yang, Lei
Yang, Guang
Sun, Xiance
author_facet Bai, Jie
Yao, Xiaofeng
Jiang, Liping
Zhang, Qiaoting
Guan, Huai
Liu, Shuang
Wu, Wei
Qiu, Tianming
Gao, Ni
Yang, Lei
Yang, Guang
Sun, Xiance
author_sort Bai, Jie
collection PubMed
description Chronic exposures to arsenic had been associated with metabolism diseases. Peroxisome proliferator-activated receptor gamma (PPARγ) was found in the liver, regulated metabolism. Here, we found that the expression of PPARγ was decreased, the generation of reactive oxygen species (ROS) and autophagy were increased after treatment with As(2)O(3) in offsprings’ livers. Taurine (Tau), a sulfur-containing β–amino acid could reverse As(2)O(3)-inhibited PPARγ. Tau also inhibit the generation of ROS and autophagy. We also found that As(2)O(3) caused autophagic cell death and ROS accelerated in HepG2 cells. Before incubation with As(2)O(3), the cells were pretreated with PPARγ activator Rosiglitazone (RGS), we found that autophagy and ROS was inhibited in HepG2 cells, suggesting that inhibition of PPARγ contributed to As(2)O(3)-induced autophagy and the generation of ROS. After pretreatment with Tau, the level of PPARγ was improved and the autophagy and ROS was inhibited in As(2)O(3)-treated cells, suggesting that Tau could protect hepatocytes against As(2)O(3) through modulating PPARγ pathway.
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spelling pubmed-49042132016-06-14 Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway Bai, Jie Yao, Xiaofeng Jiang, Liping Zhang, Qiaoting Guan, Huai Liu, Shuang Wu, Wei Qiu, Tianming Gao, Ni Yang, Lei Yang, Guang Sun, Xiance Sci Rep Article Chronic exposures to arsenic had been associated with metabolism diseases. Peroxisome proliferator-activated receptor gamma (PPARγ) was found in the liver, regulated metabolism. Here, we found that the expression of PPARγ was decreased, the generation of reactive oxygen species (ROS) and autophagy were increased after treatment with As(2)O(3) in offsprings’ livers. Taurine (Tau), a sulfur-containing β–amino acid could reverse As(2)O(3)-inhibited PPARγ. Tau also inhibit the generation of ROS and autophagy. We also found that As(2)O(3) caused autophagic cell death and ROS accelerated in HepG2 cells. Before incubation with As(2)O(3), the cells were pretreated with PPARγ activator Rosiglitazone (RGS), we found that autophagy and ROS was inhibited in HepG2 cells, suggesting that inhibition of PPARγ contributed to As(2)O(3)-induced autophagy and the generation of ROS. After pretreatment with Tau, the level of PPARγ was improved and the autophagy and ROS was inhibited in As(2)O(3)-treated cells, suggesting that Tau could protect hepatocytes against As(2)O(3) through modulating PPARγ pathway. Nature Publishing Group 2016-06-13 /pmc/articles/PMC4904213/ /pubmed/27291853 http://dx.doi.org/10.1038/srep27733 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bai, Jie
Yao, Xiaofeng
Jiang, Liping
Zhang, Qiaoting
Guan, Huai
Liu, Shuang
Wu, Wei
Qiu, Tianming
Gao, Ni
Yang, Lei
Yang, Guang
Sun, Xiance
Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway
title Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway
title_full Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway
title_fullStr Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway
title_full_unstemmed Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway
title_short Taurine protects against As(2)O(3)-induced autophagy in livers of rat offsprings through PPARγ pathway
title_sort taurine protects against as(2)o(3)-induced autophagy in livers of rat offsprings through pparγ pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904213/
https://www.ncbi.nlm.nih.gov/pubmed/27291853
http://dx.doi.org/10.1038/srep27733
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