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Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation

Nitrogen is essential for plant survival and growth. Excessive application of nitrogenous fertilizer has generated serious environment pollution and increased production cost in agriculture. To deal with this problem, tremendous efforts have been invested worldwide to increase the nitrogen use abili...

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Autores principales: Yu, Lin-Hui, Wu, Jie, Tang, Hui, Yuan, Yang, Wang, Shi-Mei, Wang, Yu-Ping, Zhu, Qi-Sheng, Li, Shi-Gui, Xiang, Cheng-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904239/
https://www.ncbi.nlm.nih.gov/pubmed/27293103
http://dx.doi.org/10.1038/srep27795
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author Yu, Lin-Hui
Wu, Jie
Tang, Hui
Yuan, Yang
Wang, Shi-Mei
Wang, Yu-Ping
Zhu, Qi-Sheng
Li, Shi-Gui
Xiang, Cheng-Bin
author_facet Yu, Lin-Hui
Wu, Jie
Tang, Hui
Yuan, Yang
Wang, Shi-Mei
Wang, Yu-Ping
Zhu, Qi-Sheng
Li, Shi-Gui
Xiang, Cheng-Bin
author_sort Yu, Lin-Hui
collection PubMed
description Nitrogen is essential for plant survival and growth. Excessive application of nitrogenous fertilizer has generated serious environment pollution and increased production cost in agriculture. To deal with this problem, tremendous efforts have been invested worldwide to increase the nitrogen use ability of crops. However, only limited success has been achieved to date. Here we report that NLP7 (NIN-LIKE PROTEIN 7) is a potential candidate to improve plant nitrogen use ability. When overexpressed in Arabidopsis, NLP7 increases plant biomass under both nitrogen-poor and -rich conditions with better-developed root system and reduced shoot/root ratio. NLP7–overexpressing plants show a significant increase in key nitrogen metabolites, nitrogen uptake, total nitrogen content, and expression levels of genes involved in nitrogen assimilation and signalling. More importantly, overexpression of NLP7 also enhances photosynthesis rate and carbon assimilation, whereas knockout of NLP7 impaired both nitrogen and carbon assimilation. In addition, NLP7 improves plant growth and nitrogen use in transgenic tobacco (Nicotiana tabacum). Our results demonstrate that NLP7 significantly improves plant growth under both nitrogen-poor and -rich conditions by coordinately enhancing nitrogen and carbon assimilation and sheds light on crop improvement.
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spelling pubmed-49042392016-06-14 Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation Yu, Lin-Hui Wu, Jie Tang, Hui Yuan, Yang Wang, Shi-Mei Wang, Yu-Ping Zhu, Qi-Sheng Li, Shi-Gui Xiang, Cheng-Bin Sci Rep Article Nitrogen is essential for plant survival and growth. Excessive application of nitrogenous fertilizer has generated serious environment pollution and increased production cost in agriculture. To deal with this problem, tremendous efforts have been invested worldwide to increase the nitrogen use ability of crops. However, only limited success has been achieved to date. Here we report that NLP7 (NIN-LIKE PROTEIN 7) is a potential candidate to improve plant nitrogen use ability. When overexpressed in Arabidopsis, NLP7 increases plant biomass under both nitrogen-poor and -rich conditions with better-developed root system and reduced shoot/root ratio. NLP7–overexpressing plants show a significant increase in key nitrogen metabolites, nitrogen uptake, total nitrogen content, and expression levels of genes involved in nitrogen assimilation and signalling. More importantly, overexpression of NLP7 also enhances photosynthesis rate and carbon assimilation, whereas knockout of NLP7 impaired both nitrogen and carbon assimilation. In addition, NLP7 improves plant growth and nitrogen use in transgenic tobacco (Nicotiana tabacum). Our results demonstrate that NLP7 significantly improves plant growth under both nitrogen-poor and -rich conditions by coordinately enhancing nitrogen and carbon assimilation and sheds light on crop improvement. Nature Publishing Group 2016-06-13 /pmc/articles/PMC4904239/ /pubmed/27293103 http://dx.doi.org/10.1038/srep27795 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yu, Lin-Hui
Wu, Jie
Tang, Hui
Yuan, Yang
Wang, Shi-Mei
Wang, Yu-Ping
Zhu, Qi-Sheng
Li, Shi-Gui
Xiang, Cheng-Bin
Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
title Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
title_full Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
title_fullStr Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
title_full_unstemmed Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
title_short Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
title_sort overexpression of arabidopsis nlp7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904239/
https://www.ncbi.nlm.nih.gov/pubmed/27293103
http://dx.doi.org/10.1038/srep27795
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