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Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation

Ultraviolet (UV) irradiation induces skin pigmentation, which relies on the intercellular crosstalk of melanin between melanocytes to keratinocytes. However, studying the separate effects of UVA and UVB irradiation reveals differences in cellular response. Herein, we show an immediate shedding of ex...

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Autores principales: Wäster, Petra, Eriksson, Ida, Vainikka, Linda, Rosdahl, Inger, Öllinger, Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904274/
https://www.ncbi.nlm.nih.gov/pubmed/27293048
http://dx.doi.org/10.1038/srep27890
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author Wäster, Petra
Eriksson, Ida
Vainikka, Linda
Rosdahl, Inger
Öllinger, Karin
author_facet Wäster, Petra
Eriksson, Ida
Vainikka, Linda
Rosdahl, Inger
Öllinger, Karin
author_sort Wäster, Petra
collection PubMed
description Ultraviolet (UV) irradiation induces skin pigmentation, which relies on the intercellular crosstalk of melanin between melanocytes to keratinocytes. However, studying the separate effects of UVA and UVB irradiation reveals differences in cellular response. Herein, we show an immediate shedding of extracellular vesicles (EVs) from the plasma membrane when exposing human melanocytes to UVA, but not UVB. The EV-shedding is preceded by UVA-induced plasma membrane damage, which is rapidly repaired by Ca(2+)-dependent lysosomal exocytosis. Using co-cultures of melanocytes and keratinocytes, we show that EVs are preferably endocytosed by keratinocytes. Importantly, EV-formation is prevented by the inhibition of exocytosis and increased lysosomal pH but is not affected by actin and microtubule inhibitors. Melanosome transfer from melanocytes to keratinocytes is equally stimulated by UVA and UVB and depends on a functional cytoskeleton. In conclusion, we show a novel cell response after UVA irradiation, resulting in transfer of lysosome-derived EVs from melanocytes to keratinocytes.
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spelling pubmed-49042742016-06-14 Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation Wäster, Petra Eriksson, Ida Vainikka, Linda Rosdahl, Inger Öllinger, Karin Sci Rep Article Ultraviolet (UV) irradiation induces skin pigmentation, which relies on the intercellular crosstalk of melanin between melanocytes to keratinocytes. However, studying the separate effects of UVA and UVB irradiation reveals differences in cellular response. Herein, we show an immediate shedding of extracellular vesicles (EVs) from the plasma membrane when exposing human melanocytes to UVA, but not UVB. The EV-shedding is preceded by UVA-induced plasma membrane damage, which is rapidly repaired by Ca(2+)-dependent lysosomal exocytosis. Using co-cultures of melanocytes and keratinocytes, we show that EVs are preferably endocytosed by keratinocytes. Importantly, EV-formation is prevented by the inhibition of exocytosis and increased lysosomal pH but is not affected by actin and microtubule inhibitors. Melanosome transfer from melanocytes to keratinocytes is equally stimulated by UVA and UVB and depends on a functional cytoskeleton. In conclusion, we show a novel cell response after UVA irradiation, resulting in transfer of lysosome-derived EVs from melanocytes to keratinocytes. Nature Publishing Group 2016-06-13 /pmc/articles/PMC4904274/ /pubmed/27293048 http://dx.doi.org/10.1038/srep27890 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wäster, Petra
Eriksson, Ida
Vainikka, Linda
Rosdahl, Inger
Öllinger, Karin
Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
title Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
title_full Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
title_fullStr Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
title_full_unstemmed Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
title_short Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
title_sort extracellular vesicles are transferred from melanocytes to keratinocytes after uva irradiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904274/
https://www.ncbi.nlm.nih.gov/pubmed/27293048
http://dx.doi.org/10.1038/srep27890
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