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Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro

The purpose of this study was to investigate the anti-Newcastle disease virus (NDV) activities of baicalin from Scutellaria baicalensis, a Traditional Chinese Medicine in vitro. Chicken embryo fibroblasts (CEFs) were infected with NDV, and quantitative analysis of apoptotic cells was performed using...

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Detalles Bibliográficos
Autores principales: JIA, Yan, XU, Ruiguang, HU, Yanchun, ZHU, Tianlong, MA, Tingting, WU, Haihong, HU, Liwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905837/
https://www.ncbi.nlm.nih.gov/pubmed/26902693
http://dx.doi.org/10.1292/jvms.15-0572
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author JIA, Yan
XU, Ruiguang
HU, Yanchun
ZHU, Tianlong
MA, Tingting
WU, Haihong
HU, Liwen
author_facet JIA, Yan
XU, Ruiguang
HU, Yanchun
ZHU, Tianlong
MA, Tingting
WU, Haihong
HU, Liwen
author_sort JIA, Yan
collection PubMed
description The purpose of this study was to investigate the anti-Newcastle disease virus (NDV) activities of baicalin from Scutellaria baicalensis, a Traditional Chinese Medicine in vitro. Chicken embryo fibroblasts (CEFs) were infected with NDV, and quantitative analysis of apoptotic cells was performed using flow cytometry. Cytotoxicity and anti-viral activities of baicalin were studied using the MTT method. The results showed that the maximal safe concentrations of baicalin to CEFs was 1 × 2(−2) mg/ml. Baicalin could directly kill NDV, inhibit the infectivity of NDV to CEF and block intracellular NDV. It inhibited the apoptosis of NDV-infected CEFs and suppressed the spread of NDV. These results indicate that baicalin has strong anti-NDV activity and has the potential for use as components of an antiviral drug.
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spelling pubmed-49058372016-06-15 Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro JIA, Yan XU, Ruiguang HU, Yanchun ZHU, Tianlong MA, Tingting WU, Haihong HU, Liwen J Vet Med Sci Pharmacology The purpose of this study was to investigate the anti-Newcastle disease virus (NDV) activities of baicalin from Scutellaria baicalensis, a Traditional Chinese Medicine in vitro. Chicken embryo fibroblasts (CEFs) were infected with NDV, and quantitative analysis of apoptotic cells was performed using flow cytometry. Cytotoxicity and anti-viral activities of baicalin were studied using the MTT method. The results showed that the maximal safe concentrations of baicalin to CEFs was 1 × 2(−2) mg/ml. Baicalin could directly kill NDV, inhibit the infectivity of NDV to CEF and block intracellular NDV. It inhibited the apoptosis of NDV-infected CEFs and suppressed the spread of NDV. These results indicate that baicalin has strong anti-NDV activity and has the potential for use as components of an antiviral drug. The Japanese Society of Veterinary Science 2016-02-20 2016-05 /pmc/articles/PMC4905837/ /pubmed/26902693 http://dx.doi.org/10.1292/jvms.15-0572 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Pharmacology
JIA, Yan
XU, Ruiguang
HU, Yanchun
ZHU, Tianlong
MA, Tingting
WU, Haihong
HU, Liwen
Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro
title Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro
title_full Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro
title_fullStr Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro
title_full_unstemmed Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro
title_short Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro
title_sort anti-ndv activity of baicalin from a traditional chinese medicine in vitro
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905837/
https://www.ncbi.nlm.nih.gov/pubmed/26902693
http://dx.doi.org/10.1292/jvms.15-0572
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