Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan

Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japa...

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Detalles Bibliográficos
Autores principales: LIU, Mingming, CAO, Shinuo, VUDRIKO, Patrick, SUZUKI, Hiroshi, SOMA, Takehisa, XUAN, Xuenan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905845/
https://www.ncbi.nlm.nih.gov/pubmed/26806537
http://dx.doi.org/10.1292/jvms.15-0483
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author LIU, Mingming
CAO, Shinuo
VUDRIKO, Patrick
SUZUKI, Hiroshi
SOMA, Takehisa
XUAN, Xuenan
author_facet LIU, Mingming
CAO, Shinuo
VUDRIKO, Patrick
SUZUKI, Hiroshi
SOMA, Takehisa
XUAN, Xuenan
author_sort LIU, Mingming
collection PubMed
description Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2–100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.
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spelling pubmed-49058452016-06-15 Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan LIU, Mingming CAO, Shinuo VUDRIKO, Patrick SUZUKI, Hiroshi SOMA, Takehisa XUAN, Xuenan J Vet Med Sci Parasitology Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2–100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite. The Japanese Society of Veterinary Science 2016-01-22 2016-05 /pmc/articles/PMC4905845/ /pubmed/26806537 http://dx.doi.org/10.1292/jvms.15-0483 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Parasitology
LIU, Mingming
CAO, Shinuo
VUDRIKO, Patrick
SUZUKI, Hiroshi
SOMA, Takehisa
XUAN, Xuenan
Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
title Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
title_full Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
title_fullStr Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
title_full_unstemmed Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
title_short Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
title_sort babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across japan
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905845/
https://www.ncbi.nlm.nih.gov/pubmed/26806537
http://dx.doi.org/10.1292/jvms.15-0483
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