SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells

Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), a...

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Detalles Bibliográficos
Autores principales: Roustazadeh, Abazar, Monavari, Seyed Hamidreza, Hosseini Fard, Seyed Reza, Hassanpour, Parisa, Yarnazari, Amaneh, Najafi, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906098/
https://www.ncbi.nlm.nih.gov/pubmed/27375980
http://dx.doi.org/10.1186/s40064-016-2329-8
Descripción
Sumario:Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), and rs1800801 (G/A) sites were constructed and transferred into human G292 osteoblast cells using pGL3-Basic plasmid. The reporter gene expression was calculated for the high and low frequency polymorphic haplotypes (CTG and TCA, respectively). Results showed that the reporter gene expression levels are not statistically different (p > 0.3). We concluded that the investigated polymorphic sites are not able to change the gene expression pattern in human G292 osteoblast cells.

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