SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells

Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), a...

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Autores principales: Roustazadeh, Abazar, Monavari, Seyed Hamidreza, Hosseini Fard, Seyed Reza, Hassanpour, Parisa, Yarnazari, Amaneh, Najafi, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906098/
https://www.ncbi.nlm.nih.gov/pubmed/27375980
http://dx.doi.org/10.1186/s40064-016-2329-8
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author Roustazadeh, Abazar
Monavari, Seyed Hamidreza
Hosseini Fard, Seyed Reza
Hassanpour, Parisa
Yarnazari, Amaneh
Najafi, Mohammad
author_facet Roustazadeh, Abazar
Monavari, Seyed Hamidreza
Hosseini Fard, Seyed Reza
Hassanpour, Parisa
Yarnazari, Amaneh
Najafi, Mohammad
author_sort Roustazadeh, Abazar
collection PubMed
description Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), and rs1800801 (G/A) sites were constructed and transferred into human G292 osteoblast cells using pGL3-Basic plasmid. The reporter gene expression was calculated for the high and low frequency polymorphic haplotypes (CTG and TCA, respectively). Results showed that the reporter gene expression levels are not statistically different (p > 0.3). We concluded that the investigated polymorphic sites are not able to change the gene expression pattern in human G292 osteoblast cells.
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spelling pubmed-49060982016-07-01 SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells Roustazadeh, Abazar Monavari, Seyed Hamidreza Hosseini Fard, Seyed Reza Hassanpour, Parisa Yarnazari, Amaneh Najafi, Mohammad Springerplus Research Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), and rs1800801 (G/A) sites were constructed and transferred into human G292 osteoblast cells using pGL3-Basic plasmid. The reporter gene expression was calculated for the high and low frequency polymorphic haplotypes (CTG and TCA, respectively). Results showed that the reporter gene expression levels are not statistically different (p > 0.3). We concluded that the investigated polymorphic sites are not able to change the gene expression pattern in human G292 osteoblast cells. Springer International Publishing 2016-06-13 /pmc/articles/PMC4906098/ /pubmed/27375980 http://dx.doi.org/10.1186/s40064-016-2329-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Roustazadeh, Abazar
Monavari, Seyed Hamidreza
Hosseini Fard, Seyed Reza
Hassanpour, Parisa
Yarnazari, Amaneh
Najafi, Mohammad
SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells
title SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells
title_full SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells
title_fullStr SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells
title_full_unstemmed SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells
title_short SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells
title_sort snp haplotyping technique for evaluation of mgp 5′ utr power in osteoblast cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906098/
https://www.ncbi.nlm.nih.gov/pubmed/27375980
http://dx.doi.org/10.1186/s40064-016-2329-8
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