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Internal guide RNA interactions interfere with Cas9-mediated cleavage

The CRISPR/Cas system uses guide RNAs (gRNAs) to direct sequence-specific DNA cleavage. Not every gRNA elicits cleavage and the mechanisms that govern gRNA activity have not been resolved. Low activity could result from either failure to form a functional Cas9–gRNA complex or inability to recognize...

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Detalles Bibliográficos
Autores principales:	Thyme, Summer B., Akhmetova, Laila, Montague, Tessa G., Valen, Eivind, Schier, Alexander F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906408/ https://www.ncbi.nlm.nih.gov/pubmed/27282953 http://dx.doi.org/10.1038/ncomms11750

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