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Increased autophagic response in a population of metastatic breast cancer cells

Breast cancer cells are heterogeneous in their ability to invade and fully metastasize, and thus also in their capacity to survive the numerous stresses encountered throughout the multiple steps of the metastatic cascade. Considering the role of autophagy as a survival response to stress, the presen...

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Autores principales: LI, YI, LIBBY, EMILY FALK, LEWIS, MONICA J., LIU, JIANZHONG, SHACKA, JOHN J., HURST, DOUGLAS R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906619/
https://www.ncbi.nlm.nih.gov/pubmed/27347175
http://dx.doi.org/10.3892/ol.2016.4613
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author LI, YI
LIBBY, EMILY FALK
LEWIS, MONICA J.
LIU, JIANZHONG
SHACKA, JOHN J.
HURST, DOUGLAS R.
author_facet LI, YI
LIBBY, EMILY FALK
LEWIS, MONICA J.
LIU, JIANZHONG
SHACKA, JOHN J.
HURST, DOUGLAS R.
author_sort LI, YI
collection PubMed
description Breast cancer cells are heterogeneous in their ability to invade and fully metastasize, and thus also in their capacity to survive the numerous stresses encountered throughout the multiple steps of the metastatic cascade. Considering the role of autophagy as a survival response to stress, the present study hypothesized that distinct populations of breast cancer cells may possess an altered autophagic capacity that influences their metastatic potential. It was observed that a metastatic breast cancer cell line, MDA-MB-231, that was sensitive to autophagic induction additionally possessed the ability to proliferate following nutrient deprivation. Furthermore, a selected subpopulation of these cells that survived multiple exposures to starvation conditions demonstrated a heightened response to autophagic induction compared to their parent cells. Although this subpopulation maintained a more grape-like pattern in three-dimensional culture compared to the extended spikes of the parent population, autophagic induction in this subpopulation elicited an invasive phenotype with extended spikes. Taken together, these results suggest that autophagic induction may contribute to the ability of distinct breast cancer cell populations to survive and invade.
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spelling pubmed-49066192016-06-24 Increased autophagic response in a population of metastatic breast cancer cells LI, YI LIBBY, EMILY FALK LEWIS, MONICA J. LIU, JIANZHONG SHACKA, JOHN J. HURST, DOUGLAS R. Oncol Lett Articles Breast cancer cells are heterogeneous in their ability to invade and fully metastasize, and thus also in their capacity to survive the numerous stresses encountered throughout the multiple steps of the metastatic cascade. Considering the role of autophagy as a survival response to stress, the present study hypothesized that distinct populations of breast cancer cells may possess an altered autophagic capacity that influences their metastatic potential. It was observed that a metastatic breast cancer cell line, MDA-MB-231, that was sensitive to autophagic induction additionally possessed the ability to proliferate following nutrient deprivation. Furthermore, a selected subpopulation of these cells that survived multiple exposures to starvation conditions demonstrated a heightened response to autophagic induction compared to their parent cells. Although this subpopulation maintained a more grape-like pattern in three-dimensional culture compared to the extended spikes of the parent population, autophagic induction in this subpopulation elicited an invasive phenotype with extended spikes. Taken together, these results suggest that autophagic induction may contribute to the ability of distinct breast cancer cell populations to survive and invade. D.A. Spandidos 2016-07 2016-05-24 /pmc/articles/PMC4906619/ /pubmed/27347175 http://dx.doi.org/10.3892/ol.2016.4613 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
LI, YI
LIBBY, EMILY FALK
LEWIS, MONICA J.
LIU, JIANZHONG
SHACKA, JOHN J.
HURST, DOUGLAS R.
Increased autophagic response in a population of metastatic breast cancer cells
title Increased autophagic response in a population of metastatic breast cancer cells
title_full Increased autophagic response in a population of metastatic breast cancer cells
title_fullStr Increased autophagic response in a population of metastatic breast cancer cells
title_full_unstemmed Increased autophagic response in a population of metastatic breast cancer cells
title_short Increased autophagic response in a population of metastatic breast cancer cells
title_sort increased autophagic response in a population of metastatic breast cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906619/
https://www.ncbi.nlm.nih.gov/pubmed/27347175
http://dx.doi.org/10.3892/ol.2016.4613
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