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The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals
Background. A loss of mucosal tolerance to the resident microbiome has been postulated in the aetiopathogenesis of spondyloarthritis, thus the purpose of these studies was to investigate microbial communities that colonise the oral cavity of patients with axial spondyloarthritis (AxSpA) and to compa...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906644/ https://www.ncbi.nlm.nih.gov/pubmed/27330858 http://dx.doi.org/10.7717/peerj.2095 |
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author | Bisanz, Jordan E. Suppiah, Praema Thomson, W. Murray Milne, Trudy Yeoh, Nigel Nolan, Anita Ettinger, Grace Reid, Gregor Gloor, Gregory B. Burton, Jeremy P. Cullinan, Mary P. Stebbings, Simon M. |
author_facet | Bisanz, Jordan E. Suppiah, Praema Thomson, W. Murray Milne, Trudy Yeoh, Nigel Nolan, Anita Ettinger, Grace Reid, Gregor Gloor, Gregory B. Burton, Jeremy P. Cullinan, Mary P. Stebbings, Simon M. |
author_sort | Bisanz, Jordan E. |
collection | PubMed |
description | Background. A loss of mucosal tolerance to the resident microbiome has been postulated in the aetiopathogenesis of spondyloarthritis, thus the purpose of these studies was to investigate microbial communities that colonise the oral cavity of patients with axial spondyloarthritis (AxSpA) and to compare these with microbial profiles of a matched healthy population. Methods. Thirty-nine participants, 17 patients with AxSpA and 22 age and gender-matched disease-free controls were recruited to the study. For patients with AxSpA, disease activity was assessed using the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). All participants underwent a detailed dental examination to assess oral health, including the presence of periodontal disease assessed using probing pocket depth (PPD). Plaque samples were obtained and their bacterial populations were profiled using Ion Torrent sequencing of the V6 region of the 16S rRNA gene. Results.Patients with AxSpA had active disease (BASDAI 4.1 ± 2.1 [mean ± SD]), and a significantly greater prevalence of periodontitis (PPD ≥ 4 mm at ≥4 sites) than controls. Bacterial communities did not differ between the two groups with multiple metrics of α and β diversity considered. Analysis of operational taxonomic units (OTUs) and higher levels of taxonomic assignment did not provide strong evidence of any single taxa associated with AxSpA in the subgingival plaque. Discussion. Although 16S rRNA gene sequencing did not identify specific bacterial profiles associated with AxSpA, there remains the potential for the microbiota to exert functional and metabolic influences in the oral cavity which could be involved in the pathogenesis of AxSpA. |
format | Online Article Text |
id | pubmed-4906644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49066442016-06-17 The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals Bisanz, Jordan E. Suppiah, Praema Thomson, W. Murray Milne, Trudy Yeoh, Nigel Nolan, Anita Ettinger, Grace Reid, Gregor Gloor, Gregory B. Burton, Jeremy P. Cullinan, Mary P. Stebbings, Simon M. PeerJ Microbiology Background. A loss of mucosal tolerance to the resident microbiome has been postulated in the aetiopathogenesis of spondyloarthritis, thus the purpose of these studies was to investigate microbial communities that colonise the oral cavity of patients with axial spondyloarthritis (AxSpA) and to compare these with microbial profiles of a matched healthy population. Methods. Thirty-nine participants, 17 patients with AxSpA and 22 age and gender-matched disease-free controls were recruited to the study. For patients with AxSpA, disease activity was assessed using the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). All participants underwent a detailed dental examination to assess oral health, including the presence of periodontal disease assessed using probing pocket depth (PPD). Plaque samples were obtained and their bacterial populations were profiled using Ion Torrent sequencing of the V6 region of the 16S rRNA gene. Results.Patients with AxSpA had active disease (BASDAI 4.1 ± 2.1 [mean ± SD]), and a significantly greater prevalence of periodontitis (PPD ≥ 4 mm at ≥4 sites) than controls. Bacterial communities did not differ between the two groups with multiple metrics of α and β diversity considered. Analysis of operational taxonomic units (OTUs) and higher levels of taxonomic assignment did not provide strong evidence of any single taxa associated with AxSpA in the subgingival plaque. Discussion. Although 16S rRNA gene sequencing did not identify specific bacterial profiles associated with AxSpA, there remains the potential for the microbiota to exert functional and metabolic influences in the oral cavity which could be involved in the pathogenesis of AxSpA. PeerJ Inc. 2016-06-09 /pmc/articles/PMC4906644/ /pubmed/27330858 http://dx.doi.org/10.7717/peerj.2095 Text en ©2016 Bisanz et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Microbiology Bisanz, Jordan E. Suppiah, Praema Thomson, W. Murray Milne, Trudy Yeoh, Nigel Nolan, Anita Ettinger, Grace Reid, Gregor Gloor, Gregory B. Burton, Jeremy P. Cullinan, Mary P. Stebbings, Simon M. The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
title | The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
title_full | The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
title_fullStr | The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
title_full_unstemmed | The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
title_short | The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
title_sort | oral microbiome of patients with axial spondyloarthritis compared to healthy individuals |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906644/ https://www.ncbi.nlm.nih.gov/pubmed/27330858 http://dx.doi.org/10.7717/peerj.2095 |
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