Evaluation of Isotope (32)P Method to Mark Culex pipiens (Diptera: Culicidae) in a Laboratory

BACKGROUND: The aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope (32)P and determine the optimal concentration of it. METHODS: An isotonic physiological saline solution, containing different concentration of radioactive isotope (32)P-labeled disodium phosphate (Na(2)H(32)PO(4)) was injected into rabbits via the jugular vein in the laboratory. Emerged Cx. pipiens were marked after feeding on rabbit. At the same time, the labeled conditions of emerged Cx. pipiens were also measured by placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside. RESULTS: According to the label condition of Cx. pipiens after taking blood and the effect of different dosage Na(2)H(32)PO(4) on rabbit health, the optimal concentration of radioactive isotope was determined, that is, 0.1211 mCi/kg. By placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside, the emerged mosquitoes were also labeled. Therefore, feeding mosquitoes on the animal injected with radioactive Na(2)H(32)PO(4) was more practical for detecting and tracing mosquitoes. CONCLUSION: The method was less time-consuming, more sensitive and safer. This marking method will facilitate post-bloodmeal studies of mosquitoes and other blood-sucking insects.