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Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients

BACKGROUND: To analyze the role of proinflammatory cytokines in drug-induced gingival enlargement in Indian population. AIM: To evaluate for the presence of interleukin-6 (IL-6) in drug-induced gingival enlargement and to compare it with healthy control in the absence of enlargement. MATERIALS AND M...

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Autor principal: Ganesh, P. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906853/
https://www.ncbi.nlm.nih.gov/pubmed/27307657
http://dx.doi.org/10.4103/0976-237X.183048
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author Ganesh, P. R.
author_facet Ganesh, P. R.
author_sort Ganesh, P. R.
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description BACKGROUND: To analyze the role of proinflammatory cytokines in drug-induced gingival enlargement in Indian population. AIM: To evaluate for the presence of interleukin-6 (IL-6) in drug-induced gingival enlargement and to compare it with healthy control in the absence of enlargement. MATERIALS AND METHODS: Thirty-five patients selected for the study and divided into control group (10) and study group (25) consisting of phenytoin (10); cyclosporin (10) and nifedipine (5) induced gingival enlargement. Gingival overgrowth index of Seymour was used to assess overgrowth and allot groups. Under LA, incisional biopsy done, tissue sample fixed in 10% formalin and immunohistochemically evaluated for the presence of IL-6 using LAB-SA method, Labeled- Streptavidin-Biotin Method (LAB-SA kit from Zymed- 2(nd) generation LAB-SA detection system, Zymed Laboratories, CA). The results of immunohistochemistry were statistically analyzed using Kruskaal–Wallis and Mann–Whitney test. RESULTS: The data obtained from immunohistochemistry assessment shows that drug-induced gingival overgrowth (DIGO) samples express more IL-6 than control group and cyclosporin expresses more IL-6 followed by phenytoin and nifedipine. CONCLUSION: Increased IL-6 expression was noticed in all three DIGO groups in comparison with control group. Among the study group, cyclosporin expressed maximum IL-6 expression followed by phenytoin and nifedipine.
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spelling pubmed-49068532016-06-15 Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients Ganesh, P. R. Contemp Clin Dent Original Article BACKGROUND: To analyze the role of proinflammatory cytokines in drug-induced gingival enlargement in Indian population. AIM: To evaluate for the presence of interleukin-6 (IL-6) in drug-induced gingival enlargement and to compare it with healthy control in the absence of enlargement. MATERIALS AND METHODS: Thirty-five patients selected for the study and divided into control group (10) and study group (25) consisting of phenytoin (10); cyclosporin (10) and nifedipine (5) induced gingival enlargement. Gingival overgrowth index of Seymour was used to assess overgrowth and allot groups. Under LA, incisional biopsy done, tissue sample fixed in 10% formalin and immunohistochemically evaluated for the presence of IL-6 using LAB-SA method, Labeled- Streptavidin-Biotin Method (LAB-SA kit from Zymed- 2(nd) generation LAB-SA detection system, Zymed Laboratories, CA). The results of immunohistochemistry were statistically analyzed using Kruskaal–Wallis and Mann–Whitney test. RESULTS: The data obtained from immunohistochemistry assessment shows that drug-induced gingival overgrowth (DIGO) samples express more IL-6 than control group and cyclosporin expresses more IL-6 followed by phenytoin and nifedipine. CONCLUSION: Increased IL-6 expression was noticed in all three DIGO groups in comparison with control group. Among the study group, cyclosporin expressed maximum IL-6 expression followed by phenytoin and nifedipine. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4906853/ /pubmed/27307657 http://dx.doi.org/10.4103/0976-237X.183048 Text en Copyright: © Contemporary Clinical Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution NonCommercial ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ganesh, P. R.
Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
title Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
title_full Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
title_fullStr Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
title_full_unstemmed Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
title_short Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
title_sort immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906853/
https://www.ncbi.nlm.nih.gov/pubmed/27307657
http://dx.doi.org/10.4103/0976-237X.183048
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