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Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model

The aim of the study was to examine the mechanism of action of Lidan Granule (LDG) for the prevention of gallstones using a guinea pig model. One hundred guinea pigs were divided into five groups randomly: control (standard diet and saline), model [lithogenic diet (LD) and saline], LDG-H (LD and 2 g...

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Autores principales: WU, XIAO, LIANG, XIAOQIANG, DU, YIJIE, ZHANG, YAN, YANG, MENG, GONG, WEIYI, LIU, BAOJUN, DONG, JINGCHENG, ZHANG, NINGXIA, ZHANG, HONGYING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906940/
https://www.ncbi.nlm.nih.gov/pubmed/27347405
http://dx.doi.org/10.3892/br.2016.672
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author WU, XIAO
LIANG, XIAOQIANG
DU, YIJIE
ZHANG, YAN
YANG, MENG
GONG, WEIYI
LIU, BAOJUN
DONG, JINGCHENG
ZHANG, NINGXIA
ZHANG, HONGYING
author_facet WU, XIAO
LIANG, XIAOQIANG
DU, YIJIE
ZHANG, YAN
YANG, MENG
GONG, WEIYI
LIU, BAOJUN
DONG, JINGCHENG
ZHANG, NINGXIA
ZHANG, HONGYING
author_sort WU, XIAO
collection PubMed
description The aim of the study was to examine the mechanism of action of Lidan Granule (LDG) for the prevention of gallstones using a guinea pig model. One hundred guinea pigs were divided into five groups randomly: control (standard diet and saline), model [lithogenic diet (LD) and saline], LDG-H (LD and 2 g/kg of LDG), LDG-L (LD and 1 g/kg of LDG), and ursodeoxycholic acid (UDCA) (LD and UDCA) as the positive control. At 6 weeks, the rate of gallstone formation and weight of the adrenal gland were recorded and serum levels of inflammatory cytokines were measured. Levels of corticotrophin-releasing hormone (CRH) in the hypothalamus, adrenocorticotropic hormone (ACTH) in the hypophysis, and serum cortisol were determined. Bile components were tested with colorimetry. At 6 weeks, the rate of gallstone formation was significantly decreased in the LDG-H (14.29%) and LDG-L (21.43%) groups compared to the model group (81.25%; P<0.01). LDG treatment decreased the serum levels of interleukin (IL)-1, IL-6, and tumor necrosis factor-α (P<0.01). LDG decreased bile cholesterol and increased bile acid and phospholipid levels in the bile (P<0.01). LDG treatment recovered the function of the hypothalamic-pituitary-adrenal (HPA) axis by increasing the expression of CRH (P<0.01) and ACTH (P<0.05). LDG made the bile less lithogenic, improved the function of the HPA axis, and regulated the expression of inflammatory cytokines for the prevention of cholelithiasis.
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spelling pubmed-49069402016-06-24 Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model WU, XIAO LIANG, XIAOQIANG DU, YIJIE ZHANG, YAN YANG, MENG GONG, WEIYI LIU, BAOJUN DONG, JINGCHENG ZHANG, NINGXIA ZHANG, HONGYING Biomed Rep Articles The aim of the study was to examine the mechanism of action of Lidan Granule (LDG) for the prevention of gallstones using a guinea pig model. One hundred guinea pigs were divided into five groups randomly: control (standard diet and saline), model [lithogenic diet (LD) and saline], LDG-H (LD and 2 g/kg of LDG), LDG-L (LD and 1 g/kg of LDG), and ursodeoxycholic acid (UDCA) (LD and UDCA) as the positive control. At 6 weeks, the rate of gallstone formation and weight of the adrenal gland were recorded and serum levels of inflammatory cytokines were measured. Levels of corticotrophin-releasing hormone (CRH) in the hypothalamus, adrenocorticotropic hormone (ACTH) in the hypophysis, and serum cortisol were determined. Bile components were tested with colorimetry. At 6 weeks, the rate of gallstone formation was significantly decreased in the LDG-H (14.29%) and LDG-L (21.43%) groups compared to the model group (81.25%; P<0.01). LDG treatment decreased the serum levels of interleukin (IL)-1, IL-6, and tumor necrosis factor-α (P<0.01). LDG decreased bile cholesterol and increased bile acid and phospholipid levels in the bile (P<0.01). LDG treatment recovered the function of the hypothalamic-pituitary-adrenal (HPA) axis by increasing the expression of CRH (P<0.01) and ACTH (P<0.05). LDG made the bile less lithogenic, improved the function of the HPA axis, and regulated the expression of inflammatory cytokines for the prevention of cholelithiasis. D.A. Spandidos 2016-07 2016-05-06 /pmc/articles/PMC4906940/ /pubmed/27347405 http://dx.doi.org/10.3892/br.2016.672 Text en Copyright: © Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
WU, XIAO
LIANG, XIAOQIANG
DU, YIJIE
ZHANG, YAN
YANG, MENG
GONG, WEIYI
LIU, BAOJUN
DONG, JINGCHENG
ZHANG, NINGXIA
ZHANG, HONGYING
Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model
title Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model
title_full Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model
title_fullStr Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model
title_full_unstemmed Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model
title_short Prevention of gallstones by Lidan Granule: Insight into underlying mechanisms using a guinea pig model
title_sort prevention of gallstones by lidan granule: insight into underlying mechanisms using a guinea pig model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906940/
https://www.ncbi.nlm.nih.gov/pubmed/27347405
http://dx.doi.org/10.3892/br.2016.672
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