Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells

A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivi...

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Detalles Bibliográficos
Autores principales: Tan, Juntao, Yang, Nuo, Hu, Zixi, Su, Jing, Zhong, Jianhong, Yang, Yang, Yu, Yating, Zhu, Jianmeng, Xue, Dabin, Huang, Yingying, Lai, Zongqiang, Huang, Yong, Lu, Xiaoling, Zhao, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907968/
https://www.ncbi.nlm.nih.gov/pubmed/27299653
http://dx.doi.org/10.1186/s11671-016-1512-8
Descripción
Sumario:A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivity and specificity of Sgc8-FSNPs were assessed using flow cytometry and fluorescence microscopy. These results showed that Sgc8-FSNPs detected leukemia cells with high sensitivity and specificity. Aptamer-modified FSNPs hold promise for sensitive and specific detection of leukemia cells. Changing the aptamer may allow the FSNPs to detect other types of cancer cells.

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