Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells

A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivi...

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Detalles Bibliográficos
Autores principales: Tan, Juntao, Yang, Nuo, Hu, Zixi, Su, Jing, Zhong, Jianhong, Yang, Yang, Yu, Yating, Zhu, Jianmeng, Xue, Dabin, Huang, Yingying, Lai, Zongqiang, Huang, Yong, Lu, Xiaoling, Zhao, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907968/
https://www.ncbi.nlm.nih.gov/pubmed/27299653
http://dx.doi.org/10.1186/s11671-016-1512-8
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author Tan, Juntao
Yang, Nuo
Hu, Zixi
Su, Jing
Zhong, Jianhong
Yang, Yang
Yu, Yating
Zhu, Jianmeng
Xue, Dabin
Huang, Yingying
Lai, Zongqiang
Huang, Yong
Lu, Xiaoling
Zhao, Yongxiang
author_facet Tan, Juntao
Yang, Nuo
Hu, Zixi
Su, Jing
Zhong, Jianhong
Yang, Yang
Yu, Yating
Zhu, Jianmeng
Xue, Dabin
Huang, Yingying
Lai, Zongqiang
Huang, Yong
Lu, Xiaoling
Zhao, Yongxiang
author_sort Tan, Juntao
collection PubMed
description A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivity and specificity of Sgc8-FSNPs were assessed using flow cytometry and fluorescence microscopy. These results showed that Sgc8-FSNPs detected leukemia cells with high sensitivity and specificity. Aptamer-modified FSNPs hold promise for sensitive and specific detection of leukemia cells. Changing the aptamer may allow the FSNPs to detect other types of cancer cells.
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spelling pubmed-49079682016-07-01 Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells Tan, Juntao Yang, Nuo Hu, Zixi Su, Jing Zhong, Jianhong Yang, Yang Yu, Yating Zhu, Jianmeng Xue, Dabin Huang, Yingying Lai, Zongqiang Huang, Yong Lu, Xiaoling Zhao, Yongxiang Nanoscale Res Lett Nano Express A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivity and specificity of Sgc8-FSNPs were assessed using flow cytometry and fluorescence microscopy. These results showed that Sgc8-FSNPs detected leukemia cells with high sensitivity and specificity. Aptamer-modified FSNPs hold promise for sensitive and specific detection of leukemia cells. Changing the aptamer may allow the FSNPs to detect other types of cancer cells. Springer US 2016-06-14 /pmc/articles/PMC4907968/ /pubmed/27299653 http://dx.doi.org/10.1186/s11671-016-1512-8 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Nano Express
Tan, Juntao
Yang, Nuo
Hu, Zixi
Su, Jing
Zhong, Jianhong
Yang, Yang
Yu, Yating
Zhu, Jianmeng
Xue, Dabin
Huang, Yingying
Lai, Zongqiang
Huang, Yong
Lu, Xiaoling
Zhao, Yongxiang
Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells
title Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells
title_full Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells
title_fullStr Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells
title_full_unstemmed Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells
title_short Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells
title_sort aptamer-functionalized fluorescent silica nanoparticles for highly sensitive detection of leukemia cells
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907968/
https://www.ncbi.nlm.nih.gov/pubmed/27299653
http://dx.doi.org/10.1186/s11671-016-1512-8
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