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Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine

Intestinal resident macrophages (Mϕs) regulate gastrointestinal homeostasis via production of an anti-inflammatory cytokine interleukin (IL)-10. Although a constant replenishment by circulating monocytes is required to maintain the pool of resident Mϕs in the colonic mucosa, the homeostatic regulati...

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Autores principales: Ochi, Takanori, Feng, Yongjia, Kitamoto, Sho, Nagao-Kitamoto, Hiroko, Kuffa, Peter, Atarashi, Koji, Honda, Kenya, Teitelbaum, Daniel H., Kamada, Nobuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4908404/
https://www.ncbi.nlm.nih.gov/pubmed/27302484
http://dx.doi.org/10.1038/srep27634
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author Ochi, Takanori
Feng, Yongjia
Kitamoto, Sho
Nagao-Kitamoto, Hiroko
Kuffa, Peter
Atarashi, Koji
Honda, Kenya
Teitelbaum, Daniel H.
Kamada, Nobuhiko
author_facet Ochi, Takanori
Feng, Yongjia
Kitamoto, Sho
Nagao-Kitamoto, Hiroko
Kuffa, Peter
Atarashi, Koji
Honda, Kenya
Teitelbaum, Daniel H.
Kamada, Nobuhiko
author_sort Ochi, Takanori
collection PubMed
description Intestinal resident macrophages (Mϕs) regulate gastrointestinal homeostasis via production of an anti-inflammatory cytokine interleukin (IL)-10. Although a constant replenishment by circulating monocytes is required to maintain the pool of resident Mϕs in the colonic mucosa, the homeostatic regulation of Mϕ in the small intestine (SI) remains unclear. Here, we demonstrate that direct stimulation by dietary amino acids regulates the homeostasis of intestinal Mϕs in the SI. Mice that received total parenteral nutrition (TPN), which deprives the animals of enteral nutrients, displayed a significant decrease of IL-10-producing Mϕs in the SI, whereas the IL-10-producing CD4(+) T cells remained intact. Likewise, enteral nutrient deprivation selectively affected the monocyte-derived F4/80(+) Mϕ population, but not non-monocytic precursor-derived CD103(+) dendritic cells. Notably, in contrast to colonic Mϕs, the replenishment of SI Mϕs and their IL-10 production were not regulated by the gut microbiota. Rather, SI Mϕs were directly regulated by dietary amino acids. Collectively, our study highlights the diet-dependent, microbiota-independent regulation of IL-10-producing resident Mϕs in the SI.
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spelling pubmed-49084042016-06-15 Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine Ochi, Takanori Feng, Yongjia Kitamoto, Sho Nagao-Kitamoto, Hiroko Kuffa, Peter Atarashi, Koji Honda, Kenya Teitelbaum, Daniel H. Kamada, Nobuhiko Sci Rep Article Intestinal resident macrophages (Mϕs) regulate gastrointestinal homeostasis via production of an anti-inflammatory cytokine interleukin (IL)-10. Although a constant replenishment by circulating monocytes is required to maintain the pool of resident Mϕs in the colonic mucosa, the homeostatic regulation of Mϕ in the small intestine (SI) remains unclear. Here, we demonstrate that direct stimulation by dietary amino acids regulates the homeostasis of intestinal Mϕs in the SI. Mice that received total parenteral nutrition (TPN), which deprives the animals of enteral nutrients, displayed a significant decrease of IL-10-producing Mϕs in the SI, whereas the IL-10-producing CD4(+) T cells remained intact. Likewise, enteral nutrient deprivation selectively affected the monocyte-derived F4/80(+) Mϕ population, but not non-monocytic precursor-derived CD103(+) dendritic cells. Notably, in contrast to colonic Mϕs, the replenishment of SI Mϕs and their IL-10 production were not regulated by the gut microbiota. Rather, SI Mϕs were directly regulated by dietary amino acids. Collectively, our study highlights the diet-dependent, microbiota-independent regulation of IL-10-producing resident Mϕs in the SI. Nature Publishing Group 2016-06-15 /pmc/articles/PMC4908404/ /pubmed/27302484 http://dx.doi.org/10.1038/srep27634 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ochi, Takanori
Feng, Yongjia
Kitamoto, Sho
Nagao-Kitamoto, Hiroko
Kuffa, Peter
Atarashi, Koji
Honda, Kenya
Teitelbaum, Daniel H.
Kamada, Nobuhiko
Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
title Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
title_full Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
title_fullStr Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
title_full_unstemmed Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
title_short Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
title_sort diet-dependent, microbiota-independent regulation of il-10-producing lamina propria macrophages in the small intestine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4908404/
https://www.ncbi.nlm.nih.gov/pubmed/27302484
http://dx.doi.org/10.1038/srep27634
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