Low impact to fixed cell processing aiming transmission electron microscopy

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was...

Descripción completa

Detalles Bibliográficos
Autores principales: Barth, Ortrud Monika, da Silva, Marcos Alexandre Nunes, Barreto-Vieira, Debora Ferreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2016
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909042/
https://www.ncbi.nlm.nih.gov/pubmed/27276186
http://dx.doi.org/10.1590/0074-02760150433
Descripción
Sumario:In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.

Ejemplares similares