Low impact to fixed cell processing aiming transmission electron microscopy

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was...

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Detalles Bibliográficos
Autores principales: Barth, Ortrud Monika, da Silva, Marcos Alexandre Nunes, Barreto-Vieira, Debora Ferreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2016
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909042/
https://www.ncbi.nlm.nih.gov/pubmed/27276186
http://dx.doi.org/10.1590/0074-02760150433
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author Barth, Ortrud Monika
da Silva, Marcos Alexandre Nunes
Barreto-Vieira, Debora Ferreira
author_facet Barth, Ortrud Monika
da Silva, Marcos Alexandre Nunes
Barreto-Vieira, Debora Ferreira
author_sort Barth, Ortrud Monika
collection PubMed
description In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.
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spelling pubmed-49090422016-06-16 Low impact to fixed cell processing aiming transmission electron microscopy Barth, Ortrud Monika da Silva, Marcos Alexandre Nunes Barreto-Vieira, Debora Ferreira Mem Inst Oswaldo Cruz Technical Note In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible. Instituto Oswaldo Cruz, Ministério da Saúde 2016-06 /pmc/articles/PMC4909042/ /pubmed/27276186 http://dx.doi.org/10.1590/0074-02760150433 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Barth, Ortrud Monika
da Silva, Marcos Alexandre Nunes
Barreto-Vieira, Debora Ferreira
Low impact to fixed cell processing aiming transmission electron microscopy
title Low impact to fixed cell processing aiming transmission electron microscopy
title_full Low impact to fixed cell processing aiming transmission electron microscopy
title_fullStr Low impact to fixed cell processing aiming transmission electron microscopy
title_full_unstemmed Low impact to fixed cell processing aiming transmission electron microscopy
title_short Low impact to fixed cell processing aiming transmission electron microscopy
title_sort low impact to fixed cell processing aiming transmission electron microscopy
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909042/
https://www.ncbi.nlm.nih.gov/pubmed/27276186
http://dx.doi.org/10.1590/0074-02760150433
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AT barretovieiradeboraferreira lowimpacttofixedcellprocessingaimingtransmissionelectronmicroscopy

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