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Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana
To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-based overexpression vector (TRBO) to express...
Autores principales: | Alkanaimsh, Salem, Karuppanan, Kalimuthu, Guerrero, Andrés, Tu, Aye M., Hashimoto, Bryce, Hwang, Min Sook, Phu, My L., Arzola, Lucas, Lebrilla, Carlito B., Dandekar, Abhaya M., Falk, Bryce W., Nandi, Somen, Rodriguez, Raymond L., McDonald, Karen A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909763/ https://www.ncbi.nlm.nih.gov/pubmed/27379103 http://dx.doi.org/10.3389/fpls.2016.00743 |
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