Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary

Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characte...

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Detalles Bibliográficos
Autores principales: Kim, Dae-Jung, Park, Chae-Won, Byambaragchaa, Munkhzaya, Kim, Shin-Kwon, Lee, Bae-Ik, Hwang, Hyung-Kyu, Myeong, Jeong-In, Hong, Sun-Mee, Kang, Myung-Hwa, Min, Kwan-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909833/
https://www.ncbi.nlm.nih.gov/pubmed/27331121
http://dx.doi.org/10.1016/j.dib.2016.05.069
Descripción
Sumario:Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary.

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