Cargando…

NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis

Stomata are highly specialized epidermal structures that control transpiration and gas exchange between plants and the environment. Signal networks underlying stomatal development have been previously uncovered but much less is known about how signals involved in stomatal development are transmitted...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Liang, Guan, Liping, Qian, Pingping, Xu, Fan, Wu, Zhongliang, Wu, Yujun, He, Kai, Gou, Xiaoping, Li, Jia, Hou, Suiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909857/
https://www.ncbi.nlm.nih.gov/pubmed/26989174
http://dx.doi.org/10.1242/dev.129098
_version_ 1782437897897508864
author Chen, Liang
Guan, Liping
Qian, Pingping
Xu, Fan
Wu, Zhongliang
Wu, Yujun
He, Kai
Gou, Xiaoping
Li, Jia
Hou, Suiwen
author_facet Chen, Liang
Guan, Liping
Qian, Pingping
Xu, Fan
Wu, Zhongliang
Wu, Yujun
He, Kai
Gou, Xiaoping
Li, Jia
Hou, Suiwen
author_sort Chen, Liang
collection PubMed
description Stomata are highly specialized epidermal structures that control transpiration and gas exchange between plants and the environment. Signal networks underlying stomatal development have been previously uncovered but much less is known about how signals involved in stomatal development are transmitted to RNA polymerase II (Pol II or RPB), which plays a central role in the transcription of mRNA coding genes. Here, we identify a partial loss-of-function mutation of the third largest subunit of nuclear DNA-dependent Pol II (NRPB3) that exhibits an increased number of stomatal lineage cells and paired stomata. Phenotypic and genetic analyses indicated that NRPB3 is not only required for correct stomatal patterning, but is also essential for stomatal differentiation. Protein-protein interaction assays showed that NRPB3 directly interacts with two basic helix-loop-helix (bHLH) transcription factors, FAMA and INDUCER OF CBF EXPRESSION1 (ICE1), indicating that NRPB3 serves as an acceptor for signals from transcription factors involved in stomatal development. Our findings highlight the surprisingly conserved activating mechanisms mediated by the third largest subunit of Pol II in eukaryotes.
format Online
Article
Text
id pubmed-4909857
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Company of Biologists Ltd
record_format MEDLINE/PubMed
spelling pubmed-49098572016-07-14 NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis Chen, Liang Guan, Liping Qian, Pingping Xu, Fan Wu, Zhongliang Wu, Yujun He, Kai Gou, Xiaoping Li, Jia Hou, Suiwen Development Research Article Stomata are highly specialized epidermal structures that control transpiration and gas exchange between plants and the environment. Signal networks underlying stomatal development have been previously uncovered but much less is known about how signals involved in stomatal development are transmitted to RNA polymerase II (Pol II or RPB), which plays a central role in the transcription of mRNA coding genes. Here, we identify a partial loss-of-function mutation of the third largest subunit of nuclear DNA-dependent Pol II (NRPB3) that exhibits an increased number of stomatal lineage cells and paired stomata. Phenotypic and genetic analyses indicated that NRPB3 is not only required for correct stomatal patterning, but is also essential for stomatal differentiation. Protein-protein interaction assays showed that NRPB3 directly interacts with two basic helix-loop-helix (bHLH) transcription factors, FAMA and INDUCER OF CBF EXPRESSION1 (ICE1), indicating that NRPB3 serves as an acceptor for signals from transcription factors involved in stomatal development. Our findings highlight the surprisingly conserved activating mechanisms mediated by the third largest subunit of Pol II in eukaryotes. The Company of Biologists Ltd 2016-05-01 /pmc/articles/PMC4909857/ /pubmed/26989174 http://dx.doi.org/10.1242/dev.129098 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Chen, Liang
Guan, Liping
Qian, Pingping
Xu, Fan
Wu, Zhongliang
Wu, Yujun
He, Kai
Gou, Xiaoping
Li, Jia
Hou, Suiwen
NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis
title NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis
title_full NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis
title_fullStr NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis
title_full_unstemmed NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis
title_short NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis
title_sort nrpb3, the third largest subunit of rna polymerase ii, is essential for stomatal patterning and differentiation in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909857/
https://www.ncbi.nlm.nih.gov/pubmed/26989174
http://dx.doi.org/10.1242/dev.129098
work_keys_str_mv AT chenliang nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT guanliping nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT qianpingping nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT xufan nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT wuzhongliang nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT wuyujun nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT hekai nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT gouxiaoping nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT lijia nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis
AT housuiwen nrpb3thethirdlargestsubunitofrnapolymeraseiiisessentialforstomatalpatterninganddifferentiationinarabidopsis