Cargando…
Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1
The metabolism of cholesterol is critical in eukaryotes as a precursor for vitamins, steroid hormones, and bile acids. Some steroid compounds can be transformed into precursors of steroid medicine by some microorganisms. In this study, the biotransformation products of cholesterol and 16α,17α-epoxyp...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909919/ https://www.ncbi.nlm.nih.gov/pubmed/27340662 http://dx.doi.org/10.1155/2016/5727631 |
_version_ | 1782437911991418880 |
---|---|
author | Zhu, XiangDong Pang, CuiPing Cao, Yuting Fan, Dan |
author_facet | Zhu, XiangDong Pang, CuiPing Cao, Yuting Fan, Dan |
author_sort | Zhu, XiangDong |
collection | PubMed |
description | The metabolism of cholesterol is critical in eukaryotes as a precursor for vitamins, steroid hormones, and bile acids. Some steroid compounds can be transformed into precursors of steroid medicine by some microorganisms. In this study, the biotransformation products of cholesterol and 16α,17α-epoxypregnenolone produced by Burkholderia cepacia SE-1 were investigated, and a correlative enzyme, hydroxylase, was also studied. The biotransformation products, 7β-hydroxycholesterol, 7-oxocholesterol, and 20-droxyl-16α,17α-epoxypregn-1,4-dien-3-one, were purified by silica gel and Sephadex LH-20 column chromatography and identified by nuclear magnetic resonance and mass spectroscopy. The hydroxylase was isolated from the bacterium and the partial sequences of the hydroxylase, which belong to the catalases/peroxidase family, were analyzed using MS/MS analyses. The enzyme showed activity toward cholesterol and had a specific activity of 37.2 U/mg of protein at 30°C and pH 7.0. |
format | Online Article Text |
id | pubmed-4909919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49099192016-06-23 Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 Zhu, XiangDong Pang, CuiPing Cao, Yuting Fan, Dan Biomed Res Int Research Article The metabolism of cholesterol is critical in eukaryotes as a precursor for vitamins, steroid hormones, and bile acids. Some steroid compounds can be transformed into precursors of steroid medicine by some microorganisms. In this study, the biotransformation products of cholesterol and 16α,17α-epoxypregnenolone produced by Burkholderia cepacia SE-1 were investigated, and a correlative enzyme, hydroxylase, was also studied. The biotransformation products, 7β-hydroxycholesterol, 7-oxocholesterol, and 20-droxyl-16α,17α-epoxypregn-1,4-dien-3-one, were purified by silica gel and Sephadex LH-20 column chromatography and identified by nuclear magnetic resonance and mass spectroscopy. The hydroxylase was isolated from the bacterium and the partial sequences of the hydroxylase, which belong to the catalases/peroxidase family, were analyzed using MS/MS analyses. The enzyme showed activity toward cholesterol and had a specific activity of 37.2 U/mg of protein at 30°C and pH 7.0. Hindawi Publishing Corporation 2016 2016-06-02 /pmc/articles/PMC4909919/ /pubmed/27340662 http://dx.doi.org/10.1155/2016/5727631 Text en Copyright © 2016 XiangDong Zhu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhu, XiangDong Pang, CuiPing Cao, Yuting Fan, Dan Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 |
title | Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 |
title_full | Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 |
title_fullStr | Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 |
title_full_unstemmed | Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 |
title_short | Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1 |
title_sort | biotransformation of cholesterol and 16α,17α-epoxypregnenolone and isolation of hydroxylase in burkholderia cepacia se-1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909919/ https://www.ncbi.nlm.nih.gov/pubmed/27340662 http://dx.doi.org/10.1155/2016/5727631 |
work_keys_str_mv | AT zhuxiangdong biotransformationofcholesteroland16a17aepoxypregnenoloneandisolationofhydroxylaseinburkholderiacepaciase1 AT pangcuiping biotransformationofcholesteroland16a17aepoxypregnenoloneandisolationofhydroxylaseinburkholderiacepaciase1 AT caoyuting biotransformationofcholesteroland16a17aepoxypregnenoloneandisolationofhydroxylaseinburkholderiacepaciase1 AT fandan biotransformationofcholesteroland16a17aepoxypregnenoloneandisolationofhydroxylaseinburkholderiacepaciase1 |