Cargando…

Scoparone affects lipid metabolism in primary hepatocytes using lipidomics

Lipidomics, which focuses on the global study of molecular lipids in biological systems, could provide valuable insights about disease mechanisms. In this study, we present a nontargeted lipidomics strategy to determine cellular lipid alterations after scoparone exposure in primary hepatocytes. Lipi...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Aihua, Qiu, Shi, Sun, Hui, Zhang, Tianlei, Guan, Yu, Han, Ying, Yan, Guangli, Wang, Xijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910084/
https://www.ncbi.nlm.nih.gov/pubmed/27306123
http://dx.doi.org/10.1038/srep28031
_version_ 1782437947393441792
author Zhang, Aihua
Qiu, Shi
Sun, Hui
Zhang, Tianlei
Guan, Yu
Han, Ying
Yan, Guangli
Wang, Xijun
author_facet Zhang, Aihua
Qiu, Shi
Sun, Hui
Zhang, Tianlei
Guan, Yu
Han, Ying
Yan, Guangli
Wang, Xijun
author_sort Zhang, Aihua
collection PubMed
description Lipidomics, which focuses on the global study of molecular lipids in biological systems, could provide valuable insights about disease mechanisms. In this study, we present a nontargeted lipidomics strategy to determine cellular lipid alterations after scoparone exposure in primary hepatocytes. Lipid metabolic profiles were analyzed by high-performance liquid chromatography coupled with time-of-flight mass spectrometry, and a novel imaging TransOmics tool has been developed for the analysis of high-resolution MS data, including the data pretreatment, visualization, automated identification, deconvolution and quantification of lipid species. Chemometric and statistical analyses of the obtained lipid fingerprints revealed the global lipidomic alterations and tested the therapeutic effects of scoparone. Identification of ten proposed lipids contributed to the better understanding of the effects of scoparone on lipid metabolism in hepatocytes. The most striking finding was that scoparone caused comprehensive lipid changes, as represented by significant changes of the identificated lipids. The levels of identified PG(19:1(9Z)/14:0), PE(17:1(9Z)/0:0), PE(19:1(9Z)/0:0) were found to be upregulated in ethanol-induced group, whereas the levels in scoparone group were downregulated. Lipid metabolism in primary hepatocytes was changed significantly by scoparone treatment. We believe that this novel approach could substantially broaden the applications of high mass resolution mass spectrometry for cellular lipidomics.
format Online
Article
Text
id pubmed-4910084
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-49100842016-06-16 Scoparone affects lipid metabolism in primary hepatocytes using lipidomics Zhang, Aihua Qiu, Shi Sun, Hui Zhang, Tianlei Guan, Yu Han, Ying Yan, Guangli Wang, Xijun Sci Rep Article Lipidomics, which focuses on the global study of molecular lipids in biological systems, could provide valuable insights about disease mechanisms. In this study, we present a nontargeted lipidomics strategy to determine cellular lipid alterations after scoparone exposure in primary hepatocytes. Lipid metabolic profiles were analyzed by high-performance liquid chromatography coupled with time-of-flight mass spectrometry, and a novel imaging TransOmics tool has been developed for the analysis of high-resolution MS data, including the data pretreatment, visualization, automated identification, deconvolution and quantification of lipid species. Chemometric and statistical analyses of the obtained lipid fingerprints revealed the global lipidomic alterations and tested the therapeutic effects of scoparone. Identification of ten proposed lipids contributed to the better understanding of the effects of scoparone on lipid metabolism in hepatocytes. The most striking finding was that scoparone caused comprehensive lipid changes, as represented by significant changes of the identificated lipids. The levels of identified PG(19:1(9Z)/14:0), PE(17:1(9Z)/0:0), PE(19:1(9Z)/0:0) were found to be upregulated in ethanol-induced group, whereas the levels in scoparone group were downregulated. Lipid metabolism in primary hepatocytes was changed significantly by scoparone treatment. We believe that this novel approach could substantially broaden the applications of high mass resolution mass spectrometry for cellular lipidomics. Nature Publishing Group 2016-06-16 /pmc/articles/PMC4910084/ /pubmed/27306123 http://dx.doi.org/10.1038/srep28031 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhang, Aihua
Qiu, Shi
Sun, Hui
Zhang, Tianlei
Guan, Yu
Han, Ying
Yan, Guangli
Wang, Xijun
Scoparone affects lipid metabolism in primary hepatocytes using lipidomics
title Scoparone affects lipid metabolism in primary hepatocytes using lipidomics
title_full Scoparone affects lipid metabolism in primary hepatocytes using lipidomics
title_fullStr Scoparone affects lipid metabolism in primary hepatocytes using lipidomics
title_full_unstemmed Scoparone affects lipid metabolism in primary hepatocytes using lipidomics
title_short Scoparone affects lipid metabolism in primary hepatocytes using lipidomics
title_sort scoparone affects lipid metabolism in primary hepatocytes using lipidomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910084/
https://www.ncbi.nlm.nih.gov/pubmed/27306123
http://dx.doi.org/10.1038/srep28031
work_keys_str_mv AT zhangaihua scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT qiushi scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT sunhui scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT zhangtianlei scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT guanyu scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT hanying scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT yanguangli scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics
AT wangxijun scoparoneaffectslipidmetabolisminprimaryhepatocytesusinglipidomics