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GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes
Podocyte foot processes are interdigitated to form the slit diaphragm and are crucial for the glomerular filtration barrier. Glucocorticoid-induced transcript 1 (GLCCI1) is transcriptionally regulated, but its signaling pathway in podocytes is unknown. The main objective of this study was to investi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910149/ https://www.ncbi.nlm.nih.gov/pubmed/27174202 http://dx.doi.org/10.1038/emm.2016.28 |
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author | Kim, Sang-Hoon Kim, Hyun-Jung Kim, Chan-Wha |
author_facet | Kim, Sang-Hoon Kim, Hyun-Jung Kim, Chan-Wha |
author_sort | Kim, Sang-Hoon |
collection | PubMed |
description | Podocyte foot processes are interdigitated to form the slit diaphragm and are crucial for the glomerular filtration barrier. Glucocorticoid-induced transcript 1 (GLCCI1) is transcriptionally regulated, but its signaling pathway in podocytes is unknown. The main objective of this study was to investigate the regulation of podocyte foot process proteins and to investigate the role of GLCCI1 in the phosphoinositide 3-kinase (PI3K) pathway using high glucose-induced podocytes and streptozotocin-induced diabetic rats. In podocytes and rat kidneys, GLCCI1 was found to be highly specific for the glomerulus and podocyte foot processes similar to other podocyte-specific proteins (nephrin, podocin, synatopodin and podocalyxin) based on reverse transcription-PCR, western blotting, immunofluorescence and immunoelectron microscopy analyses. In addition, the decrease in the GLCCI1 expression level under hyperglycemic conditions was restored by treatment with a PI3K inhibitor (wortmannin). Immunofluorescence analysis confirmed that GLCCI1 colocalized with nephrin and synaptopodin both in vivo and in vitro. Finally, immunoelectron microscopy data from streptozotocin-induced diabetic rats showed that GLCCI1 also localized in podocyte foot processes. Hence, GLCCI1 is a component of podocyte foot processes, and its expression appears to be regulated via the PI3K pathway. |
format | Online Article Text |
id | pubmed-4910149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49101492016-06-23 GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes Kim, Sang-Hoon Kim, Hyun-Jung Kim, Chan-Wha Exp Mol Med Original Article Podocyte foot processes are interdigitated to form the slit diaphragm and are crucial for the glomerular filtration barrier. Glucocorticoid-induced transcript 1 (GLCCI1) is transcriptionally regulated, but its signaling pathway in podocytes is unknown. The main objective of this study was to investigate the regulation of podocyte foot process proteins and to investigate the role of GLCCI1 in the phosphoinositide 3-kinase (PI3K) pathway using high glucose-induced podocytes and streptozotocin-induced diabetic rats. In podocytes and rat kidneys, GLCCI1 was found to be highly specific for the glomerulus and podocyte foot processes similar to other podocyte-specific proteins (nephrin, podocin, synatopodin and podocalyxin) based on reverse transcription-PCR, western blotting, immunofluorescence and immunoelectron microscopy analyses. In addition, the decrease in the GLCCI1 expression level under hyperglycemic conditions was restored by treatment with a PI3K inhibitor (wortmannin). Immunofluorescence analysis confirmed that GLCCI1 colocalized with nephrin and synaptopodin both in vivo and in vitro. Finally, immunoelectron microscopy data from streptozotocin-induced diabetic rats showed that GLCCI1 also localized in podocyte foot processes. Hence, GLCCI1 is a component of podocyte foot processes, and its expression appears to be regulated via the PI3K pathway. Nature Publishing Group 2016-05 2016-05-13 /pmc/articles/PMC4910149/ /pubmed/27174202 http://dx.doi.org/10.1038/emm.2016.28 Text en Copyright © 2016 KSBMB. http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Original Article Kim, Sang-Hoon Kim, Hyun-Jung Kim, Chan-Wha GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes |
title | GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes |
title_full | GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes |
title_fullStr | GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes |
title_full_unstemmed | GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes |
title_short | GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes |
title_sort | glcci1 is a novel component associated with the pi3k signaling pathway in podocyte foot processes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910149/ https://www.ncbi.nlm.nih.gov/pubmed/27174202 http://dx.doi.org/10.1038/emm.2016.28 |
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