Cargando…
Detection of chikungunya virus in saliva and urine
BACKGROUND: Saliva and urine have been used for arthropod-borne viruses molecular detection but not yet for chikungunya virus (CHIKV). We investigated the use of saliva and urine for molecular detection of CHIKV during the French Polynesian outbreak. METHODS: During the French Polynesian chikungunya...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910250/ https://www.ncbi.nlm.nih.gov/pubmed/27306056 http://dx.doi.org/10.1186/s12985-016-0556-9 |
_version_ | 1782437980808413184 |
---|---|
author | Musso, Didier Teissier, Anita Rouault, Eline Teururai, Sylviane de Pina, Jean-Jacques Nhan, Tu-Xuan |
author_facet | Musso, Didier Teissier, Anita Rouault, Eline Teururai, Sylviane de Pina, Jean-Jacques Nhan, Tu-Xuan |
author_sort | Musso, Didier |
collection | PubMed |
description | BACKGROUND: Saliva and urine have been used for arthropod-borne viruses molecular detection but not yet for chikungunya virus (CHIKV). We investigated the use of saliva and urine for molecular detection of CHIKV during the French Polynesian outbreak. METHODS: During the French Polynesian chikungunya outbreak (2014–2015), we collected the same day blood and saliva samples from 60 patients with probable chikungunya (47 during the 1st week post symptoms onset and 13 after), urine was available for 39 of them. All samples were tested using a CHIKV reverse-transcription PCR. RESULTS: Forty eight patients had confirmed chikungunya. For confirmed chikungunya presenting during the 1st week post symptoms onset, CHIKV RNA was detected from 86.1 % (31/36) of blood, 58.3 % (21/36) of saliva and 8.3 % (2/24) of urine. Detection rate of CHIKV RNA was significantly higher in blood compared to saliva. For confirmed chikungunya presenting after the 1st week post symptoms onset, CHIKV RNA was detected from 8.3 % (1/12) of blood, 8.3 % (1/12) of saliva and 0 % (0/8) of urine. CONCLUSIONS: In contrast to Zika virus (ZIKV), saliva did not increased the detection rate of CHIKV RNA during the 1st week post symptoms onset. In contrast to ZIKV, dengue virus and West Nile virus, urine did not enlarged the window of detection of CHIKV RNA after the 1st week post symptoms onset. Saliva can be used for molecular detection of CHIKV during the 1st week post symptoms onset only if blood is impossible to collect but with a lower sensitivity compared to blood. |
format | Online Article Text |
id | pubmed-4910250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49102502016-06-17 Detection of chikungunya virus in saliva and urine Musso, Didier Teissier, Anita Rouault, Eline Teururai, Sylviane de Pina, Jean-Jacques Nhan, Tu-Xuan Virol J Short Report BACKGROUND: Saliva and urine have been used for arthropod-borne viruses molecular detection but not yet for chikungunya virus (CHIKV). We investigated the use of saliva and urine for molecular detection of CHIKV during the French Polynesian outbreak. METHODS: During the French Polynesian chikungunya outbreak (2014–2015), we collected the same day blood and saliva samples from 60 patients with probable chikungunya (47 during the 1st week post symptoms onset and 13 after), urine was available for 39 of them. All samples were tested using a CHIKV reverse-transcription PCR. RESULTS: Forty eight patients had confirmed chikungunya. For confirmed chikungunya presenting during the 1st week post symptoms onset, CHIKV RNA was detected from 86.1 % (31/36) of blood, 58.3 % (21/36) of saliva and 8.3 % (2/24) of urine. Detection rate of CHIKV RNA was significantly higher in blood compared to saliva. For confirmed chikungunya presenting after the 1st week post symptoms onset, CHIKV RNA was detected from 8.3 % (1/12) of blood, 8.3 % (1/12) of saliva and 0 % (0/8) of urine. CONCLUSIONS: In contrast to Zika virus (ZIKV), saliva did not increased the detection rate of CHIKV RNA during the 1st week post symptoms onset. In contrast to ZIKV, dengue virus and West Nile virus, urine did not enlarged the window of detection of CHIKV RNA after the 1st week post symptoms onset. Saliva can be used for molecular detection of CHIKV during the 1st week post symptoms onset only if blood is impossible to collect but with a lower sensitivity compared to blood. BioMed Central 2016-06-16 /pmc/articles/PMC4910250/ /pubmed/27306056 http://dx.doi.org/10.1186/s12985-016-0556-9 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Musso, Didier Teissier, Anita Rouault, Eline Teururai, Sylviane de Pina, Jean-Jacques Nhan, Tu-Xuan Detection of chikungunya virus in saliva and urine |
title | Detection of chikungunya virus in saliva and urine |
title_full | Detection of chikungunya virus in saliva and urine |
title_fullStr | Detection of chikungunya virus in saliva and urine |
title_full_unstemmed | Detection of chikungunya virus in saliva and urine |
title_short | Detection of chikungunya virus in saliva and urine |
title_sort | detection of chikungunya virus in saliva and urine |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910250/ https://www.ncbi.nlm.nih.gov/pubmed/27306056 http://dx.doi.org/10.1186/s12985-016-0556-9 |
work_keys_str_mv | AT mussodidier detectionofchikungunyavirusinsalivaandurine AT teissieranita detectionofchikungunyavirusinsalivaandurine AT rouaulteline detectionofchikungunyavirusinsalivaandurine AT teururaisylviane detectionofchikungunyavirusinsalivaandurine AT depinajeanjacques detectionofchikungunyavirusinsalivaandurine AT nhantuxuan detectionofchikungunyavirusinsalivaandurine |