Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart

This data article contains the data related to the research article ‘Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated β-adrenoceptor activation-induced cardiac hypertrophic growth in experimental rats’ (Venkatesan et al., 2016...

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Detalles Bibliográficos
Autores principales: Venkatesan, Balaji, Tumala, Anusha, Subramanian, Vimala, Vellaichamy, Elangovan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910299/
https://www.ncbi.nlm.nih.gov/pubmed/27366782
http://dx.doi.org/10.1016/j.dib.2016.05.074
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author Venkatesan, Balaji
Tumala, Anusha
Subramanian, Vimala
Vellaichamy, Elangovan
author_facet Venkatesan, Balaji
Tumala, Anusha
Subramanian, Vimala
Vellaichamy, Elangovan
author_sort Venkatesan, Balaji
collection PubMed
description This data article contains the data related to the research article ‘Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated β-adrenoceptor activation-induced cardiac hypertrophic growth in experimental rats’ (Venkatesan et al., 2016 [1]). The siRNA-Npr3 loaded chitosan nanoparticles were synthesized using ionotropic gelation method, where the positive charge of the chitosan interacts with the negative charge of STPP and siRNA-Npr3. The physicochemical properties of the synthesized siRNA-Npr3 loaded chitosan nanoparticles were studied by dynamic light scattering, FE-SEM and HR-TEM analysis. In addition, the loading efficiency and stability of the nanoparticles were also studied. Further, the gene silencing efficacy, hemocompatibility and biocompatibility were studied using Wistar rats (in vivo), isolated red blood cells and H9c2 cardiomyoblast cells, respectively.
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spelling pubmed-49102992016-06-30 Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart Venkatesan, Balaji Tumala, Anusha Subramanian, Vimala Vellaichamy, Elangovan Data Brief Data Article This data article contains the data related to the research article ‘Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated β-adrenoceptor activation-induced cardiac hypertrophic growth in experimental rats’ (Venkatesan et al., 2016 [1]). The siRNA-Npr3 loaded chitosan nanoparticles were synthesized using ionotropic gelation method, where the positive charge of the chitosan interacts with the negative charge of STPP and siRNA-Npr3. The physicochemical properties of the synthesized siRNA-Npr3 loaded chitosan nanoparticles were studied by dynamic light scattering, FE-SEM and HR-TEM analysis. In addition, the loading efficiency and stability of the nanoparticles were also studied. Further, the gene silencing efficacy, hemocompatibility and biocompatibility were studied using Wistar rats (in vivo), isolated red blood cells and H9c2 cardiomyoblast cells, respectively. Elsevier 2016-06-03 /pmc/articles/PMC4910299/ /pubmed/27366782 http://dx.doi.org/10.1016/j.dib.2016.05.074 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Venkatesan, Balaji
Tumala, Anusha
Subramanian, Vimala
Vellaichamy, Elangovan
Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart
title Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart
title_full Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart
title_fullStr Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart
title_full_unstemmed Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart
title_short Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart
title_sort data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of sirna-npr3: targeting npr-c expression in the heart
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910299/
https://www.ncbi.nlm.nih.gov/pubmed/27366782
http://dx.doi.org/10.1016/j.dib.2016.05.074
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