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A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology

Rapid diagnostic tests (RDTs) can detect anti-malaria antibodies in human blood. As they can detect parasite infection at the low parasite density, they are useful in endemic areas where light infection and/or re-infection of parasites are common. Thus, malaria antibody tests can be used for screeni...

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Autores principales: Cho, Sung Jin, Lee, Jihoo, Lee, Hyun Jae, Jo, Hyun-Young, Sinniah, Mangalam, Kim, Hak-Yong, Chong, Chom-Kyu, Song, Hyun-Ok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910601/
https://www.ncbi.nlm.nih.gov/pubmed/27313496
http://dx.doi.org/10.7150/ijbs.14408
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author Cho, Sung Jin
Lee, Jihoo
Lee, Hyun Jae
Jo, Hyun-Young
Sinniah, Mangalam
Kim, Hak-Yong
Chong, Chom-Kyu
Song, Hyun-Ok
author_facet Cho, Sung Jin
Lee, Jihoo
Lee, Hyun Jae
Jo, Hyun-Young
Sinniah, Mangalam
Kim, Hak-Yong
Chong, Chom-Kyu
Song, Hyun-Ok
author_sort Cho, Sung Jin
collection PubMed
description Rapid diagnostic tests (RDTs) can detect anti-malaria antibodies in human blood. As they can detect parasite infection at the low parasite density, they are useful in endemic areas where light infection and/or re-infection of parasites are common. Thus, malaria antibody tests can be used for screening bloods in blood banks to prevent transfusion-transmitted malaria (TTM), an emerging problem in malaria endemic areas. However, only a few malaria antibody tests are available in the microwell-based assay format and these are not suitable for field application. A novel malaria antibody (Ab)-based RDT using a differential diagnostic marker for falciparum and vivax malaria was developed as a suitable high-throughput assay that is sensitive and practical for blood screening. The marker, merozoite surface protein 1 (MSP1) was discovered by generation of a Plasmodium-specific network and the hierarchical organization of modularity in the network. Clinical evaluation revealed that the novel Malaria Pf/Pv Ab RDT shows improved sensitivity (98%) and specificity (99.7%) compared with the performance of a commercial kit, SD BioLine Malaria P.f/P.v (95.1% sensitivity and 99.1% specificity). The novel Malaria Pf/Pv Ab RDT has potential for use as a cost-effective blood-screening tool for malaria and in turn, reduces TTM risk in endemic areas.
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spelling pubmed-49106012016-06-16 A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology Cho, Sung Jin Lee, Jihoo Lee, Hyun Jae Jo, Hyun-Young Sinniah, Mangalam Kim, Hak-Yong Chong, Chom-Kyu Song, Hyun-Ok Int J Biol Sci Research Paper Rapid diagnostic tests (RDTs) can detect anti-malaria antibodies in human blood. As they can detect parasite infection at the low parasite density, they are useful in endemic areas where light infection and/or re-infection of parasites are common. Thus, malaria antibody tests can be used for screening bloods in blood banks to prevent transfusion-transmitted malaria (TTM), an emerging problem in malaria endemic areas. However, only a few malaria antibody tests are available in the microwell-based assay format and these are not suitable for field application. A novel malaria antibody (Ab)-based RDT using a differential diagnostic marker for falciparum and vivax malaria was developed as a suitable high-throughput assay that is sensitive and practical for blood screening. The marker, merozoite surface protein 1 (MSP1) was discovered by generation of a Plasmodium-specific network and the hierarchical organization of modularity in the network. Clinical evaluation revealed that the novel Malaria Pf/Pv Ab RDT shows improved sensitivity (98%) and specificity (99.7%) compared with the performance of a commercial kit, SD BioLine Malaria P.f/P.v (95.1% sensitivity and 99.1% specificity). The novel Malaria Pf/Pv Ab RDT has potential for use as a cost-effective blood-screening tool for malaria and in turn, reduces TTM risk in endemic areas. Ivyspring International Publisher 2016-05-25 /pmc/articles/PMC4910601/ /pubmed/27313496 http://dx.doi.org/10.7150/ijbs.14408 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Cho, Sung Jin
Lee, Jihoo
Lee, Hyun Jae
Jo, Hyun-Young
Sinniah, Mangalam
Kim, Hak-Yong
Chong, Chom-Kyu
Song, Hyun-Ok
A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
title A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
title_full A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
title_fullStr A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
title_full_unstemmed A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
title_short A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
title_sort novel malaria pf/pv ab rapid diagnostic test using a differential diagnostic marker identified by network biology
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910601/
https://www.ncbi.nlm.nih.gov/pubmed/27313496
http://dx.doi.org/10.7150/ijbs.14408
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