Cargando…
Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila
Mating and gametogenesis are two essential components of animal reproduction. Gametogenesis must be modulated by the need for gametes, yet little is known of how mating, a process that utilizes gametes, may modulate the process of gametogenesis. Here, we report that mating stimulates female germline...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911108/ https://www.ncbi.nlm.nih.gov/pubmed/27310920 http://dx.doi.org/10.1371/journal.pgen.1006123 |
_version_ | 1782438087722270720 |
---|---|
author | Ameku, Tomotsune Niwa, Ryusuke |
author_facet | Ameku, Tomotsune Niwa, Ryusuke |
author_sort | Ameku, Tomotsune |
collection | PubMed |
description | Mating and gametogenesis are two essential components of animal reproduction. Gametogenesis must be modulated by the need for gametes, yet little is known of how mating, a process that utilizes gametes, may modulate the process of gametogenesis. Here, we report that mating stimulates female germline stem cell (GSC) proliferation in Drosophila melanogaster. Mating-induced increase in GSC number is not simply owing to the indirect effect of emission of stored eggs, but rather is stimulated by a male-derived Sex Peptide (SP) and its receptor SPR, the components of a canonical neuronal pathway that induces a post-mating behavioral switch in females. We show that ecdysteroid, the major insect steroid hormone, regulates mating-induced GSC proliferation independently of insulin signaling. Ovarian ecdysteroid level increases after mating and transmits its signal directly through the ecdysone receptor expressed in the ovarian niche to increase the number of GSCs. Impairment of ovarian ecdysteroid biosynthesis disrupts mating-induced increase in GSCs as well as egg production. Importantly, feeding of ecdysteroid rescues the decrease in GSC number caused by impairment of neuronal SP signaling. Our study illustrates how female GSC activity is coordinately regulated by the neuroendocrine system to sustain reproductive success in response to mating. |
format | Online Article Text |
id | pubmed-4911108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49111082016-07-06 Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila Ameku, Tomotsune Niwa, Ryusuke PLoS Genet Research Article Mating and gametogenesis are two essential components of animal reproduction. Gametogenesis must be modulated by the need for gametes, yet little is known of how mating, a process that utilizes gametes, may modulate the process of gametogenesis. Here, we report that mating stimulates female germline stem cell (GSC) proliferation in Drosophila melanogaster. Mating-induced increase in GSC number is not simply owing to the indirect effect of emission of stored eggs, but rather is stimulated by a male-derived Sex Peptide (SP) and its receptor SPR, the components of a canonical neuronal pathway that induces a post-mating behavioral switch in females. We show that ecdysteroid, the major insect steroid hormone, regulates mating-induced GSC proliferation independently of insulin signaling. Ovarian ecdysteroid level increases after mating and transmits its signal directly through the ecdysone receptor expressed in the ovarian niche to increase the number of GSCs. Impairment of ovarian ecdysteroid biosynthesis disrupts mating-induced increase in GSCs as well as egg production. Importantly, feeding of ecdysteroid rescues the decrease in GSC number caused by impairment of neuronal SP signaling. Our study illustrates how female GSC activity is coordinately regulated by the neuroendocrine system to sustain reproductive success in response to mating. Public Library of Science 2016-06-16 /pmc/articles/PMC4911108/ /pubmed/27310920 http://dx.doi.org/10.1371/journal.pgen.1006123 Text en © 2016 Ameku, Niwa http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ameku, Tomotsune Niwa, Ryusuke Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila |
title | Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila |
title_full | Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila |
title_fullStr | Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila |
title_full_unstemmed | Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila |
title_short | Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila |
title_sort | mating-induced increase in germline stem cells via the neuroendocrine system in female drosophila |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911108/ https://www.ncbi.nlm.nih.gov/pubmed/27310920 http://dx.doi.org/10.1371/journal.pgen.1006123 |
work_keys_str_mv | AT amekutomotsune matinginducedincreaseingermlinestemcellsviatheneuroendocrinesysteminfemaledrosophila AT niwaryusuke matinginducedincreaseingermlinestemcellsviatheneuroendocrinesysteminfemaledrosophila |