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The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies

BACKGROUND: In renal biopsy reporting, quantitative measurements, such as glomerular number and percentage of globally sclerotic glomeruli, is central to diagnostic accuracy and prognosis. The aim of this study is to determine the number of glomeruli and percent globally sclerotic in renal biopsies...

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Autores principales: Rosenberg, Avi Z., Palmer, Matthew, Merlino, Lino, Troost, Jonathan P., Gasim, Adil, Bagnasco, Serena, Avila-Casado, Carmen, Johnstone, Duncan, Hodgin, Jeffrey B., Conway, Catherine, Gillespie, Brenda W., Nast, Cynthia C., Barisoni, Laura, Hewitt, Stephen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911144/
https://www.ncbi.nlm.nih.gov/pubmed/27310011
http://dx.doi.org/10.1371/journal.pone.0156441
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author Rosenberg, Avi Z.
Palmer, Matthew
Merlino, Lino
Troost, Jonathan P.
Gasim, Adil
Bagnasco, Serena
Avila-Casado, Carmen
Johnstone, Duncan
Hodgin, Jeffrey B.
Conway, Catherine
Gillespie, Brenda W.
Nast, Cynthia C.
Barisoni, Laura
Hewitt, Stephen M.
author_facet Rosenberg, Avi Z.
Palmer, Matthew
Merlino, Lino
Troost, Jonathan P.
Gasim, Adil
Bagnasco, Serena
Avila-Casado, Carmen
Johnstone, Duncan
Hodgin, Jeffrey B.
Conway, Catherine
Gillespie, Brenda W.
Nast, Cynthia C.
Barisoni, Laura
Hewitt, Stephen M.
author_sort Rosenberg, Avi Z.
collection PubMed
description BACKGROUND: In renal biopsy reporting, quantitative measurements, such as glomerular number and percentage of globally sclerotic glomeruli, is central to diagnostic accuracy and prognosis. The aim of this study is to determine the number of glomeruli and percent globally sclerotic in renal biopsies by means of registration of serial tissue sections and manual enumeration, compared to the numbers in pathology reports from routine light microscopic assessment. DESIGN: We reviewed 277 biopsies from the Nephrotic Syndrome Study Network (NEPTUNE) digital pathology repository, enumerating 9,379 glomeruli by means of whole slide imaging. Glomerular number and the percentage of globally sclerotic glomeruli are values routinely recorded in the official renal biopsy pathology report from the 25 participating centers. Two general trends in reporting were noted: total number per biopsy or average number per level/section. Both of these approaches were assessed for their accuracy in comparison to the analogous numbers of annotated glomeruli on WSI. RESULTS: The number of glomeruli annotated was consistently higher than those reported (p<0.001); this difference was proportional to the number of glomeruli. In contrast, percent globally sclerotic were similar when calculated on total glomeruli, but greater in FSGS when calculated on average number of glomeruli (p<0.01). The difference in percent globally sclerotic between annotated and those recorded in pathology reports was significant when global sclerosis is greater than 40%. CONCLUSIONS: Although glass slides were not available for direct comparison to whole slide image annotation, this study indicates that routine manual light microscopy assessment of number of glomeruli is inaccurate, and the magnitude of this error is proportional to the total number of glomeruli.
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spelling pubmed-49111442016-07-06 The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies Rosenberg, Avi Z. Palmer, Matthew Merlino, Lino Troost, Jonathan P. Gasim, Adil Bagnasco, Serena Avila-Casado, Carmen Johnstone, Duncan Hodgin, Jeffrey B. Conway, Catherine Gillespie, Brenda W. Nast, Cynthia C. Barisoni, Laura Hewitt, Stephen M. PLoS One Research Article BACKGROUND: In renal biopsy reporting, quantitative measurements, such as glomerular number and percentage of globally sclerotic glomeruli, is central to diagnostic accuracy and prognosis. The aim of this study is to determine the number of glomeruli and percent globally sclerotic in renal biopsies by means of registration of serial tissue sections and manual enumeration, compared to the numbers in pathology reports from routine light microscopic assessment. DESIGN: We reviewed 277 biopsies from the Nephrotic Syndrome Study Network (NEPTUNE) digital pathology repository, enumerating 9,379 glomeruli by means of whole slide imaging. Glomerular number and the percentage of globally sclerotic glomeruli are values routinely recorded in the official renal biopsy pathology report from the 25 participating centers. Two general trends in reporting were noted: total number per biopsy or average number per level/section. Both of these approaches were assessed for their accuracy in comparison to the analogous numbers of annotated glomeruli on WSI. RESULTS: The number of glomeruli annotated was consistently higher than those reported (p<0.001); this difference was proportional to the number of glomeruli. In contrast, percent globally sclerotic were similar when calculated on total glomeruli, but greater in FSGS when calculated on average number of glomeruli (p<0.01). The difference in percent globally sclerotic between annotated and those recorded in pathology reports was significant when global sclerosis is greater than 40%. CONCLUSIONS: Although glass slides were not available for direct comparison to whole slide image annotation, this study indicates that routine manual light microscopy assessment of number of glomeruli is inaccurate, and the magnitude of this error is proportional to the total number of glomeruli. Public Library of Science 2016-06-16 /pmc/articles/PMC4911144/ /pubmed/27310011 http://dx.doi.org/10.1371/journal.pone.0156441 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Rosenberg, Avi Z.
Palmer, Matthew
Merlino, Lino
Troost, Jonathan P.
Gasim, Adil
Bagnasco, Serena
Avila-Casado, Carmen
Johnstone, Duncan
Hodgin, Jeffrey B.
Conway, Catherine
Gillespie, Brenda W.
Nast, Cynthia C.
Barisoni, Laura
Hewitt, Stephen M.
The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies
title The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies
title_full The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies
title_fullStr The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies
title_full_unstemmed The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies
title_short The Application of Digital Pathology to Improve Accuracy in Glomerular Enumeration in Renal Biopsies
title_sort application of digital pathology to improve accuracy in glomerular enumeration in renal biopsies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911144/
https://www.ncbi.nlm.nih.gov/pubmed/27310011
http://dx.doi.org/10.1371/journal.pone.0156441
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