Protein crystallization screens developed at the MRC Laboratory of Molecular Biology

In order to solve increasingly challenging protein structures with crystallography, crystallization reagents and screen formulations are regularly investigated. Here, we briefly describe 96-condition screens developed at the MRC Laboratory of Molecular Biology: the LMB sparse matrix screen, Pi incom...

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Autor principal: Gorrec, Fabrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science Ltd. ;, Distributed by Virgin Mailing and Distribution 2016
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911435/
https://www.ncbi.nlm.nih.gov/pubmed/27032894
http://dx.doi.org/10.1016/j.drudis.2016.03.008
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author Gorrec, Fabrice
author_facet Gorrec, Fabrice
author_sort Gorrec, Fabrice
collection PubMed
description In order to solve increasingly challenging protein structures with crystallography, crystallization reagents and screen formulations are regularly investigated. Here, we briefly describe 96-condition screens developed at the MRC Laboratory of Molecular Biology: the LMB sparse matrix screen, Pi incomplete factorial screens, the MORPHEUS grid screens and the ANGSTROM optimization screen. In this short review, we also discuss the difficulties and advantages associated with the development of protein crystallization screens.
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spelling pubmed-49114352016-06-26 Protein crystallization screens developed at the MRC Laboratory of Molecular Biology Gorrec, Fabrice Drug Discov Today Review In order to solve increasingly challenging protein structures with crystallography, crystallization reagents and screen formulations are regularly investigated. Here, we briefly describe 96-condition screens developed at the MRC Laboratory of Molecular Biology: the LMB sparse matrix screen, Pi incomplete factorial screens, the MORPHEUS grid screens and the ANGSTROM optimization screen. In this short review, we also discuss the difficulties and advantages associated with the development of protein crystallization screens. Elsevier Science Ltd. ;, Distributed by Virgin Mailing and Distribution 2016-05 /pmc/articles/PMC4911435/ /pubmed/27032894 http://dx.doi.org/10.1016/j.drudis.2016.03.008 Text en © 2016 MRC Laboratory of Molecular Biology http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gorrec, Fabrice
Protein crystallization screens developed at the MRC Laboratory of Molecular Biology
title Protein crystallization screens developed at the MRC Laboratory of Molecular Biology
title_full Protein crystallization screens developed at the MRC Laboratory of Molecular Biology
title_fullStr Protein crystallization screens developed at the MRC Laboratory of Molecular Biology
title_full_unstemmed Protein crystallization screens developed at the MRC Laboratory of Molecular Biology
title_short Protein crystallization screens developed at the MRC Laboratory of Molecular Biology
title_sort protein crystallization screens developed at the mrc laboratory of molecular biology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911435/
https://www.ncbi.nlm.nih.gov/pubmed/27032894
http://dx.doi.org/10.1016/j.drudis.2016.03.008
work_keys_str_mv AT gorrecfabrice proteincrystallizationscreensdevelopedatthemrclaboratoryofmolecularbiology

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