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Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean
Calcium ion is an intracellular messenger that plays a central role in signal transduction pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) signal network have shown different functions in the Ca(2+) signaling process. In this work, we identified the entire so...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911590/ https://www.ncbi.nlm.nih.gov/pubmed/27311690 http://dx.doi.org/10.1038/srep28225 |
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author | Zhu, Kaikai Chen, Fei Liu, Jinyi Chen, Xinlu Hewezi, Tarek Cheng, Zong-Ming (Max) |
author_facet | Zhu, Kaikai Chen, Fei Liu, Jinyi Chen, Xinlu Hewezi, Tarek Cheng, Zong-Ming (Max) |
author_sort | Zhu, Kaikai |
collection | PubMed |
description | Calcium ion is an intracellular messenger that plays a central role in signal transduction pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) signal network have shown different functions in the Ca(2+) signaling process. In this work, we identified the entire soybean (Glycine max) CIPK gene family, which comprised 52 genes and divided into four subgroups (I to IV) based on phylogeny. The gene structural analysis separated these 52 genes into an intron-rich clade and an intron-poor clade. Chromosomal location analysis resulted in the identification of 22 duplicated blocks and six tandem duplication events. Phylogenetic classification of 193 CIPK proteins from representative plant species suggested that the intron-poor clade of CIPKs originated in seed plants. Analysis of global gene expression patterns of soybean CIPK family revealed that most intron-poor soybean CIPK genes are drought-inducible; a finding that was further confirmed using qRT-PCR. Our study provides a foundation for further functional analysis to reveal the roles that CIPKs and more specifically the intron-poor clade play in drought tolerance in soybean. |
format | Online Article Text |
id | pubmed-4911590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49115902016-06-17 Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean Zhu, Kaikai Chen, Fei Liu, Jinyi Chen, Xinlu Hewezi, Tarek Cheng, Zong-Ming (Max) Sci Rep Article Calcium ion is an intracellular messenger that plays a central role in signal transduction pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) signal network have shown different functions in the Ca(2+) signaling process. In this work, we identified the entire soybean (Glycine max) CIPK gene family, which comprised 52 genes and divided into four subgroups (I to IV) based on phylogeny. The gene structural analysis separated these 52 genes into an intron-rich clade and an intron-poor clade. Chromosomal location analysis resulted in the identification of 22 duplicated blocks and six tandem duplication events. Phylogenetic classification of 193 CIPK proteins from representative plant species suggested that the intron-poor clade of CIPKs originated in seed plants. Analysis of global gene expression patterns of soybean CIPK family revealed that most intron-poor soybean CIPK genes are drought-inducible; a finding that was further confirmed using qRT-PCR. Our study provides a foundation for further functional analysis to reveal the roles that CIPKs and more specifically the intron-poor clade play in drought tolerance in soybean. Nature Publishing Group 2016-06-17 /pmc/articles/PMC4911590/ /pubmed/27311690 http://dx.doi.org/10.1038/srep28225 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhu, Kaikai Chen, Fei Liu, Jinyi Chen, Xinlu Hewezi, Tarek Cheng, Zong-Ming (Max) Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean |
title | Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean |
title_full | Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean |
title_fullStr | Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean |
title_full_unstemmed | Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean |
title_short | Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean |
title_sort | evolution of an intron-poor cluster of the cipk gene family and expression in response to drought stress in soybean |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911590/ https://www.ncbi.nlm.nih.gov/pubmed/27311690 http://dx.doi.org/10.1038/srep28225 |
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