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High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material

Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic mono...

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Autores principales: Loeffler, Felix F., Foertsch, Tobias C., Popov, Roman, Mattes, Daniela S., Schlageter, Martin, Sedlmayr, Martyna, Ridder, Barbara, Dang, Florian-Xuan, von Bojničić-Kninski, Clemens, Weber, Laura K., Fischer, Andrea, Greifenstein, Juliane, Bykovskaya, Valentina, Buliev, Ivan, Bischoff, F. Ralf, Hahn, Lothar, Meier, Michael A. R., Bräse, Stefan, Powell, Annie K., Balaban, Teodor Silviu, Breitling, Frank, Nesterov-Mueller, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911634/
https://www.ncbi.nlm.nih.gov/pubmed/27296868
http://dx.doi.org/10.1038/ncomms11844
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author Loeffler, Felix F.
Foertsch, Tobias C.
Popov, Roman
Mattes, Daniela S.
Schlageter, Martin
Sedlmayr, Martyna
Ridder, Barbara
Dang, Florian-Xuan
von Bojničić-Kninski, Clemens
Weber, Laura K.
Fischer, Andrea
Greifenstein, Juliane
Bykovskaya, Valentina
Buliev, Ivan
Bischoff, F. Ralf
Hahn, Lothar
Meier, Michael A. R.
Bräse, Stefan
Powell, Annie K.
Balaban, Teodor Silviu
Breitling, Frank
Nesterov-Mueller, Alexander
author_facet Loeffler, Felix F.
Foertsch, Tobias C.
Popov, Roman
Mattes, Daniela S.
Schlageter, Martin
Sedlmayr, Martyna
Ridder, Barbara
Dang, Florian-Xuan
von Bojničić-Kninski, Clemens
Weber, Laura K.
Fischer, Andrea
Greifenstein, Juliane
Bykovskaya, Valentina
Buliev, Ivan
Bischoff, F. Ralf
Hahn, Lothar
Meier, Michael A. R.
Bräse, Stefan
Powell, Annie K.
Balaban, Teodor Silviu
Breitling, Frank
Nesterov-Mueller, Alexander
author_sort Loeffler, Felix F.
collection PubMed
description Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array's peptides with >17,000 spots per cm(2).
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spelling pubmed-49116342016-06-29 High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material Loeffler, Felix F. Foertsch, Tobias C. Popov, Roman Mattes, Daniela S. Schlageter, Martin Sedlmayr, Martyna Ridder, Barbara Dang, Florian-Xuan von Bojničić-Kninski, Clemens Weber, Laura K. Fischer, Andrea Greifenstein, Juliane Bykovskaya, Valentina Buliev, Ivan Bischoff, F. Ralf Hahn, Lothar Meier, Michael A. R. Bräse, Stefan Powell, Annie K. Balaban, Teodor Silviu Breitling, Frank Nesterov-Mueller, Alexander Nat Commun Article Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array's peptides with >17,000 spots per cm(2). Nature Publishing Group 2016-06-14 /pmc/articles/PMC4911634/ /pubmed/27296868 http://dx.doi.org/10.1038/ncomms11844 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Loeffler, Felix F.
Foertsch, Tobias C.
Popov, Roman
Mattes, Daniela S.
Schlageter, Martin
Sedlmayr, Martyna
Ridder, Barbara
Dang, Florian-Xuan
von Bojničić-Kninski, Clemens
Weber, Laura K.
Fischer, Andrea
Greifenstein, Juliane
Bykovskaya, Valentina
Buliev, Ivan
Bischoff, F. Ralf
Hahn, Lothar
Meier, Michael A. R.
Bräse, Stefan
Powell, Annie K.
Balaban, Teodor Silviu
Breitling, Frank
Nesterov-Mueller, Alexander
High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
title High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
title_full High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
title_fullStr High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
title_full_unstemmed High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
title_short High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
title_sort high-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911634/
https://www.ncbi.nlm.nih.gov/pubmed/27296868
http://dx.doi.org/10.1038/ncomms11844
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