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Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan

Hairy vetch (Vicia villosa Roth) is a leguminous crop widely used as green manure and a cover crop in Japan. It exhibits strong weed-suppressing activity, high resistance to insect pests, and the ability to fix nitrogen through symbiotic interactions with soil bacteria known as rhizobia. Few studies...

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Autores principales: Yuan, Kun, Miwa, Hiroki, Iizuka, Maki, Yokoyama, Tadashi, Fujii, Yoshiharu, Okazaki, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912146/
https://www.ncbi.nlm.nih.gov/pubmed/27151657
http://dx.doi.org/10.1264/jsme2.ME15184
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author Yuan, Kun
Miwa, Hiroki
Iizuka, Maki
Yokoyama, Tadashi
Fujii, Yoshiharu
Okazaki, Shin
author_facet Yuan, Kun
Miwa, Hiroki
Iizuka, Maki
Yokoyama, Tadashi
Fujii, Yoshiharu
Okazaki, Shin
author_sort Yuan, Kun
collection PubMed
description Hairy vetch (Vicia villosa Roth) is a leguminous crop widely used as green manure and a cover crop in Japan. It exhibits strong weed-suppressing activity, high resistance to insect pests, and the ability to fix nitrogen through symbiotic interactions with soil bacteria known as rhizobia. Few studies have investigated the rhizobia that form nodules on hairy vetch in Japan, and the biological resources available for selecting high nitrogen-fixing rhizobia are limited. In the present study, we isolated 110 hairy vetch rhizobia from 13 different areas in Japan. Based on their 16S rRNA gene sequences, 73% of the isolates were identified as Rhizobium leguminosarum. A comparative analysis of nodC and 16S rRNA gene phylogenies revealed that several isolates possessed congruent nodC sequences despite having divergent 16S rRNA gene sequences, suggesting that the horizontal transfer of nod genes occurred during the evolution of rhizobia. Inoculation tests showed that isolates closely related to R. leguminosarum had better plant growth-promoting effects than other strains, thereby providing a promising agricultural resource for inoculating crops.
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spelling pubmed-49121462016-06-29 Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan Yuan, Kun Miwa, Hiroki Iizuka, Maki Yokoyama, Tadashi Fujii, Yoshiharu Okazaki, Shin Microbes Environ Articles Hairy vetch (Vicia villosa Roth) is a leguminous crop widely used as green manure and a cover crop in Japan. It exhibits strong weed-suppressing activity, high resistance to insect pests, and the ability to fix nitrogen through symbiotic interactions with soil bacteria known as rhizobia. Few studies have investigated the rhizobia that form nodules on hairy vetch in Japan, and the biological resources available for selecting high nitrogen-fixing rhizobia are limited. In the present study, we isolated 110 hairy vetch rhizobia from 13 different areas in Japan. Based on their 16S rRNA gene sequences, 73% of the isolates were identified as Rhizobium leguminosarum. A comparative analysis of nodC and 16S rRNA gene phylogenies revealed that several isolates possessed congruent nodC sequences despite having divergent 16S rRNA gene sequences, suggesting that the horizontal transfer of nod genes occurred during the evolution of rhizobia. Inoculation tests showed that isolates closely related to R. leguminosarum had better plant growth-promoting effects than other strains, thereby providing a promising agricultural resource for inoculating crops. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-06 2016-05-03 /pmc/articles/PMC4912146/ /pubmed/27151657 http://dx.doi.org/10.1264/jsme2.ME15184 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Yuan, Kun
Miwa, Hiroki
Iizuka, Maki
Yokoyama, Tadashi
Fujii, Yoshiharu
Okazaki, Shin
Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan
title Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan
title_full Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan
title_fullStr Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan
title_full_unstemmed Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan
title_short Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan
title_sort genetic diversity and symbiotic phenotype of hairy vetch rhizobia in japan
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912146/
https://www.ncbi.nlm.nih.gov/pubmed/27151657
http://dx.doi.org/10.1264/jsme2.ME15184
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