Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis

The plant growth-promoting fungus (PGPF), Penicillium simplicissimum GP17-2 (GP17-2), induces systemic resistance against Pseudomonas syringae pv. tomato DC3000 (Pst) in Arabidopsis thaliana. The molecular mechanisms underlying induced systemic resistance (ISR) by GP17-2 were investigated in the pre...

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Autores principales: Hieno, Ayaka, Naznin, Hushna Ara, Hyakumachi, Mitsuro, Higuchi-Takeuchi, Mieko, Matsui, Minami, Yamamoto, Yoshiharu Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912150/
https://www.ncbi.nlm.nih.gov/pubmed/27301421
http://dx.doi.org/10.1264/jsme2.ME16025
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author Hieno, Ayaka
Naznin, Hushna Ara
Hyakumachi, Mitsuro
Higuchi-Takeuchi, Mieko
Matsui, Minami
Yamamoto, Yoshiharu Y.
author_facet Hieno, Ayaka
Naznin, Hushna Ara
Hyakumachi, Mitsuro
Higuchi-Takeuchi, Mieko
Matsui, Minami
Yamamoto, Yoshiharu Y.
author_sort Hieno, Ayaka
collection PubMed
description The plant growth-promoting fungus (PGPF), Penicillium simplicissimum GP17-2 (GP17-2), induces systemic resistance against Pseudomonas syringae pv. tomato DC3000 (Pst) in Arabidopsis thaliana. The molecular mechanisms underlying induced systemic resistance (ISR) by GP17-2 were investigated in the present study. Microscopic observations revealed that stomatal reopening by Pst was restricted by elicitation with the culture filtrate (CF) from GP17-2. A gene expression analysis of MYB44, which enhances abscisic acid signaling and consequently closes stomata, revealed that the gene was activated by CF. CF-elicited myb44 mutant plants failed to restrict stomatal reopening and showed lower resistance to Pst than wild-type plants. These results indicate that stomatal resistance by GP17-2 is mediated by the gene activation of MYB44. We herein revealed that the MYB44-mediated prevention of penetration through the stomata is one of the components responsible for GP17-2-elicited ISR.
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spelling pubmed-49121502016-06-29 Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis Hieno, Ayaka Naznin, Hushna Ara Hyakumachi, Mitsuro Higuchi-Takeuchi, Mieko Matsui, Minami Yamamoto, Yoshiharu Y. Microbes Environ Articles The plant growth-promoting fungus (PGPF), Penicillium simplicissimum GP17-2 (GP17-2), induces systemic resistance against Pseudomonas syringae pv. tomato DC3000 (Pst) in Arabidopsis thaliana. The molecular mechanisms underlying induced systemic resistance (ISR) by GP17-2 were investigated in the present study. Microscopic observations revealed that stomatal reopening by Pst was restricted by elicitation with the culture filtrate (CF) from GP17-2. A gene expression analysis of MYB44, which enhances abscisic acid signaling and consequently closes stomata, revealed that the gene was activated by CF. CF-elicited myb44 mutant plants failed to restrict stomatal reopening and showed lower resistance to Pst than wild-type plants. These results indicate that stomatal resistance by GP17-2 is mediated by the gene activation of MYB44. We herein revealed that the MYB44-mediated prevention of penetration through the stomata is one of the components responsible for GP17-2-elicited ISR. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-06 2016-06-10 /pmc/articles/PMC4912150/ /pubmed/27301421 http://dx.doi.org/10.1264/jsme2.ME16025 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Hieno, Ayaka
Naznin, Hushna Ara
Hyakumachi, Mitsuro
Higuchi-Takeuchi, Mieko
Matsui, Minami
Yamamoto, Yoshiharu Y.
Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis
title Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis
title_full Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis
title_fullStr Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis
title_full_unstemmed Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis
title_short Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis
title_sort possible involvement of myb44-mediated stomatal regulation in systemic resistance induced by penicillium simplicissimum gp17-2 in arabidopsis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912150/
https://www.ncbi.nlm.nih.gov/pubmed/27301421
http://dx.doi.org/10.1264/jsme2.ME16025
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