The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707

A genome analysis of Pseudomonas pseudoalcaligenes KF707, a PCBs degrader and metal-resistant soil microorganism, revealed the presence of two novel gene clusters named che2 and che3, which were predicted to be involved in chemotaxis-like pathways, in addition to a che1 gene cluster. We herein repor...

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Autores principales: Fedi, Stefano, Barberi, Tania Triscari, Nappi, Maria Rosaria, Sandri, Federica, Booth, Sean, Turner, Raymond J., Attimonelli, Marcella, Cappelletti, Martina, Zannoni, Davide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912153/
https://www.ncbi.nlm.nih.gov/pubmed/27151656
http://dx.doi.org/10.1264/jsme2.ME15164
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author Fedi, Stefano
Barberi, Tania Triscari
Nappi, Maria Rosaria
Sandri, Federica
Booth, Sean
Turner, Raymond J.
Attimonelli, Marcella
Cappelletti, Martina
Zannoni, Davide
author_facet Fedi, Stefano
Barberi, Tania Triscari
Nappi, Maria Rosaria
Sandri, Federica
Booth, Sean
Turner, Raymond J.
Attimonelli, Marcella
Cappelletti, Martina
Zannoni, Davide
author_sort Fedi, Stefano
collection PubMed
description A genome analysis of Pseudomonas pseudoalcaligenes KF707, a PCBs degrader and metal-resistant soil microorganism, revealed the presence of two novel gene clusters named che2 and che3, which were predicted to be involved in chemotaxis-like pathways, in addition to a che1 gene cluster. We herein report that the histidine kinase coding genes, cheA2 and cheA3, have no role in swimming or chemotaxis in P. pseudoalcaligenes KF707, in contrast to cheA1. However, the cheA1 and cheA2 genes were both necessary for cell swarming, whereas the cheA3 gene product had a negative effect on the optimal swarming phenotype of KF707 cells.
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spelling pubmed-49121532016-06-29 The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707 Fedi, Stefano Barberi, Tania Triscari Nappi, Maria Rosaria Sandri, Federica Booth, Sean Turner, Raymond J. Attimonelli, Marcella Cappelletti, Martina Zannoni, Davide Microbes Environ Short Communication A genome analysis of Pseudomonas pseudoalcaligenes KF707, a PCBs degrader and metal-resistant soil microorganism, revealed the presence of two novel gene clusters named che2 and che3, which were predicted to be involved in chemotaxis-like pathways, in addition to a che1 gene cluster. We herein report that the histidine kinase coding genes, cheA2 and cheA3, have no role in swimming or chemotaxis in P. pseudoalcaligenes KF707, in contrast to cheA1. However, the cheA1 and cheA2 genes were both necessary for cell swarming, whereas the cheA3 gene product had a negative effect on the optimal swarming phenotype of KF707 cells. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-06 2016-05-03 /pmc/articles/PMC4912153/ /pubmed/27151656 http://dx.doi.org/10.1264/jsme2.ME15164 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Fedi, Stefano
Barberi, Tania Triscari
Nappi, Maria Rosaria
Sandri, Federica
Booth, Sean
Turner, Raymond J.
Attimonelli, Marcella
Cappelletti, Martina
Zannoni, Davide
The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707
title The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707
title_full The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707
title_fullStr The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707
title_full_unstemmed The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707
title_short The Role of cheA Genes in Swarming and Swimming Motility of Pseudomonas pseudoalcaligenes KF707
title_sort role of chea genes in swarming and swimming motility of pseudomonas pseudoalcaligenes kf707
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912153/
https://www.ncbi.nlm.nih.gov/pubmed/27151656
http://dx.doi.org/10.1264/jsme2.ME15164
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