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Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan
Dent corn Andisol at the Hokkaido University Shizunai Livestock Experimental Farm actively emits nitrous oxide (N(2)O). In order to screen for culturable and active N(2)O emitters with high N(2)O emission potential, soft gel medium containing excess KNO(3) was inoculated with soil suspensions from f...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912161/ https://www.ncbi.nlm.nih.gov/pubmed/27109825 http://dx.doi.org/10.1264/jsme2.ME15155 |
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author | Nie, Yanxia Li, Li Isoda, Reika Wang, Mengcen Hatano, Ryusuke Hashidoko, Yasuyuki |
author_facet | Nie, Yanxia Li, Li Isoda, Reika Wang, Mengcen Hatano, Ryusuke Hashidoko, Yasuyuki |
author_sort | Nie, Yanxia |
collection | PubMed |
description | Dent corn Andisol at the Hokkaido University Shizunai Livestock Experimental Farm actively emits nitrous oxide (N(2)O). In order to screen for culturable and active N(2)O emitters with high N(2)O emission potential, soft gel medium containing excess KNO(3) was inoculated with soil suspensions from farm soil samples collected at different land managements. Dominant bacterial colonies were searched for among 20 of the actively N(2)O-emitting cultures from post-harvest soil and 19 from pre-tilled soil, and all isolates were subjected to the culture-based N(2)O emission assay. Ten active N(2)O-emitting bacteria, four from post-harvest soil and six from pre-tilled soil, out of 156 isolates were identified as genus Pseudomonas by 16S rRNA gene sequencing. These N(2)O emitters showed clear responses to NO(3)(−) within a neutral pH range (5.5–6.7), and accelerated N(2)O production with 1.5–15 mM sucrose supplementation, suggesting the production of N(2)O during the denitrification process. However, the negative responses of 6 active N(2)O emitters, 3 from post-harvest soil and 3 from pre-tilled soil, out of the 10 isolates in the acetylene-blocking assay suggest that these 6 N(2)O emitters are incomplete denitrifiers that have lost their N(2)O reductase (N(2)OR) activity. Although the PCR assay for the denitrification-associated genes, narG and nirK/S, was positive in all 10 Pseudomonas isolates, those negative in the acetylene-blocking assay were nosZ-negative. Therefore, these results imply that the high N(2)O emission potential of dent corn Andisol is partly attributed to saprophytic, nosZ gene-missing pseudomonad denitrifiers. |
format | Online Article Text |
id | pubmed-4912161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-49121612016-06-29 Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan Nie, Yanxia Li, Li Isoda, Reika Wang, Mengcen Hatano, Ryusuke Hashidoko, Yasuyuki Microbes Environ Articles Dent corn Andisol at the Hokkaido University Shizunai Livestock Experimental Farm actively emits nitrous oxide (N(2)O). In order to screen for culturable and active N(2)O emitters with high N(2)O emission potential, soft gel medium containing excess KNO(3) was inoculated with soil suspensions from farm soil samples collected at different land managements. Dominant bacterial colonies were searched for among 20 of the actively N(2)O-emitting cultures from post-harvest soil and 19 from pre-tilled soil, and all isolates were subjected to the culture-based N(2)O emission assay. Ten active N(2)O-emitting bacteria, four from post-harvest soil and six from pre-tilled soil, out of 156 isolates were identified as genus Pseudomonas by 16S rRNA gene sequencing. These N(2)O emitters showed clear responses to NO(3)(−) within a neutral pH range (5.5–6.7), and accelerated N(2)O production with 1.5–15 mM sucrose supplementation, suggesting the production of N(2)O during the denitrification process. However, the negative responses of 6 active N(2)O emitters, 3 from post-harvest soil and 3 from pre-tilled soil, out of the 10 isolates in the acetylene-blocking assay suggest that these 6 N(2)O emitters are incomplete denitrifiers that have lost their N(2)O reductase (N(2)OR) activity. Although the PCR assay for the denitrification-associated genes, narG and nirK/S, was positive in all 10 Pseudomonas isolates, those negative in the acetylene-blocking assay were nosZ-negative. Therefore, these results imply that the high N(2)O emission potential of dent corn Andisol is partly attributed to saprophytic, nosZ gene-missing pseudomonad denitrifiers. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-06 2016-04-22 /pmc/articles/PMC4912161/ /pubmed/27109825 http://dx.doi.org/10.1264/jsme2.ME15155 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Nie, Yanxia Li, Li Isoda, Reika Wang, Mengcen Hatano, Ryusuke Hashidoko, Yasuyuki Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan |
title | Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan |
title_full | Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan |
title_fullStr | Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan |
title_full_unstemmed | Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan |
title_short | Physiological and Genotypic Characteristics of Nitrous Oxide (N2O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan |
title_sort | physiological and genotypic characteristics of nitrous oxide (n2o)-emitting pseudomonas species isolated from dent corn andisol farmland in hokkaido, japan |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912161/ https://www.ncbi.nlm.nih.gov/pubmed/27109825 http://dx.doi.org/10.1264/jsme2.ME15155 |
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