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Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma sa...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912278/ https://www.ncbi.nlm.nih.gov/pubmed/26559284 http://dx.doi.org/10.1097/MD.0000000000001988 |
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author | Zhu, Guoyan Yin, Changlin Tian, Zhu Wang, Tinggang Sun, Wei Xiang, Qiang Guo, Guoning |
author_facet | Zhu, Guoyan Yin, Changlin Tian, Zhu Wang, Tinggang Sun, Wei Xiang, Qiang Guo, Guoning |
author_sort | Zhu, Guoyan |
collection | PubMed |
description | Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma samples from 12 patients with AMS and 12 individuals without AMS were collected and used for further bioinformatics analysis by gas chromatography–mass spectrometry (GC–MS). The following analytical methods were used: gas chromatography–mass spectrometry data preprocessing, principal components analysis, partial least squares-discriminant analysis, model validation, orthogonal partial least squares-discriminant analysis, and the screening and identification of differences in metabolites. The results revealed a significant difference between the subjects with AMS and those in the control group. Compared with plasma from the controls, plasma from the AMS patients contained significantly increased hypoxanthine, cysteinylglycine, D-arabitol, L-allothreonine, 2-ketobutyric acid, and succinate semialdehyde. The identification of metabolomic changes may be useful for the diagnosis of AMS in the future and may lay the foundation for further study of AMS pathogenesis. |
format | Online Article Text |
id | pubmed-4912278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-49122782016-06-28 Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry Zhu, Guoyan Yin, Changlin Tian, Zhu Wang, Tinggang Sun, Wei Xiang, Qiang Guo, Guoning Medicine (Baltimore) 5100 Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma samples from 12 patients with AMS and 12 individuals without AMS were collected and used for further bioinformatics analysis by gas chromatography–mass spectrometry (GC–MS). The following analytical methods were used: gas chromatography–mass spectrometry data preprocessing, principal components analysis, partial least squares-discriminant analysis, model validation, orthogonal partial least squares-discriminant analysis, and the screening and identification of differences in metabolites. The results revealed a significant difference between the subjects with AMS and those in the control group. Compared with plasma from the controls, plasma from the AMS patients contained significantly increased hypoxanthine, cysteinylglycine, D-arabitol, L-allothreonine, 2-ketobutyric acid, and succinate semialdehyde. The identification of metabolomic changes may be useful for the diagnosis of AMS in the future and may lay the foundation for further study of AMS pathogenesis. Wolters Kluwer Health 2015-11-13 /pmc/articles/PMC4912278/ /pubmed/26559284 http://dx.doi.org/10.1097/MD.0000000000001988 Text en Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 |
spellingShingle | 5100 Zhu, Guoyan Yin, Changlin Tian, Zhu Wang, Tinggang Sun, Wei Xiang, Qiang Guo, Guoning Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry |
title | Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry |
title_full | Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry |
title_fullStr | Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry |
title_full_unstemmed | Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry |
title_short | Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry |
title_sort | metabolomic analysis of plasma from patients with acute mountain sickness using chromatography–mass spectrometry |
topic | 5100 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912278/ https://www.ncbi.nlm.nih.gov/pubmed/26559284 http://dx.doi.org/10.1097/MD.0000000000001988 |
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