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Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry

Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma sa...

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Autores principales: Zhu, Guoyan, Yin, Changlin, Tian, Zhu, Wang, Tinggang, Sun, Wei, Xiang, Qiang, Guo, Guoning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912278/
https://www.ncbi.nlm.nih.gov/pubmed/26559284
http://dx.doi.org/10.1097/MD.0000000000001988
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author Zhu, Guoyan
Yin, Changlin
Tian, Zhu
Wang, Tinggang
Sun, Wei
Xiang, Qiang
Guo, Guoning
author_facet Zhu, Guoyan
Yin, Changlin
Tian, Zhu
Wang, Tinggang
Sun, Wei
Xiang, Qiang
Guo, Guoning
author_sort Zhu, Guoyan
collection PubMed
description Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma samples from 12 patients with AMS and 12 individuals without AMS were collected and used for further bioinformatics analysis by gas chromatography–mass spectrometry (GC–MS). The following analytical methods were used: gas chromatography–mass spectrometry data preprocessing, principal components analysis, partial least squares-discriminant analysis, model validation, orthogonal partial least squares-discriminant analysis, and the screening and identification of differences in metabolites. The results revealed a significant difference between the subjects with AMS and those in the control group. Compared with plasma from the controls, plasma from the AMS patients contained significantly increased hypoxanthine, cysteinylglycine, D-arabitol, L-allothreonine, 2-ketobutyric acid, and succinate semialdehyde. The identification of metabolomic changes may be useful for the diagnosis of AMS in the future and may lay the foundation for further study of AMS pathogenesis.
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spelling pubmed-49122782016-06-28 Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry Zhu, Guoyan Yin, Changlin Tian, Zhu Wang, Tinggang Sun, Wei Xiang, Qiang Guo, Guoning Medicine (Baltimore) 5100 Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma samples from 12 patients with AMS and 12 individuals without AMS were collected and used for further bioinformatics analysis by gas chromatography–mass spectrometry (GC–MS). The following analytical methods were used: gas chromatography–mass spectrometry data preprocessing, principal components analysis, partial least squares-discriminant analysis, model validation, orthogonal partial least squares-discriminant analysis, and the screening and identification of differences in metabolites. The results revealed a significant difference between the subjects with AMS and those in the control group. Compared with plasma from the controls, plasma from the AMS patients contained significantly increased hypoxanthine, cysteinylglycine, D-arabitol, L-allothreonine, 2-ketobutyric acid, and succinate semialdehyde. The identification of metabolomic changes may be useful for the diagnosis of AMS in the future and may lay the foundation for further study of AMS pathogenesis. Wolters Kluwer Health 2015-11-13 /pmc/articles/PMC4912278/ /pubmed/26559284 http://dx.doi.org/10.1097/MD.0000000000001988 Text en Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0
spellingShingle 5100
Zhu, Guoyan
Yin, Changlin
Tian, Zhu
Wang, Tinggang
Sun, Wei
Xiang, Qiang
Guo, Guoning
Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
title Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
title_full Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
title_fullStr Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
title_full_unstemmed Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
title_short Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography–Mass Spectrometry
title_sort metabolomic analysis of plasma from patients with acute mountain sickness using chromatography–mass spectrometry
topic 5100
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912278/
https://www.ncbi.nlm.nih.gov/pubmed/26559284
http://dx.doi.org/10.1097/MD.0000000000001988
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