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Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells
Tumor cell vasculogenic mimicry (VM), a newly defined pattern of tumor blood supply, signifies the functional plasticity of aggressive cancer cells forming vascular networks. VM and cancer stem cells (CSCs) have been shown to be associated with tumor growth, local invasion, and distant metastasis. I...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913546/ https://www.ncbi.nlm.nih.gov/pubmed/27366084 http://dx.doi.org/10.2147/OTT.S93015 |
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author | Liu, Ying Sun, Baocun Liu, Tieju Zhao, Xiulan Wang, Xudong Li, Yanlei Meng, Jie Gu, Qiang Liu, Fang Dong, Xueyi Liu, Peimei Sun, Ran Zhao, Nan |
author_facet | Liu, Ying Sun, Baocun Liu, Tieju Zhao, Xiulan Wang, Xudong Li, Yanlei Meng, Jie Gu, Qiang Liu, Fang Dong, Xueyi Liu, Peimei Sun, Ran Zhao, Nan |
author_sort | Liu, Ying |
collection | PubMed |
description | Tumor cell vasculogenic mimicry (VM), a newly defined pattern of tumor blood supply, signifies the functional plasticity of aggressive cancer cells forming vascular networks. VM and cancer stem cells (CSCs) have been shown to be associated with tumor growth, local invasion, and distant metastasis. In our previous study, CSCs in triple-negative breast cancer were potential to participate in VM formation. In this study, breast CSCs were isolated from the triple-negative breast cancer cell line MDA-MB-231 by using mammosphere culture. Western blotting and reverse transcription polymerase chain reaction showed that mammosphere cells displayed an increased expression of AURKA protein kinase and stem cell marker c-myc and sox2. The VM formation by mammosphere cells was inhibited by AURKA knockdown or the addition of AURKA inhibitor MLN8237. In the meantime, MLN8237 induced the increased E-cadherin and decreased c-myc, sox2, and β-catenin expressions. The function of AURKA in VM formation was further confirmed using a xenograft-murine model. The results suggested that AURKA protein kinase is involved in VM formation of CSCs and may become a new treatment target in suppressing VM and metastasis of breast cancer. |
format | Online Article Text |
id | pubmed-4913546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49135462016-06-30 Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells Liu, Ying Sun, Baocun Liu, Tieju Zhao, Xiulan Wang, Xudong Li, Yanlei Meng, Jie Gu, Qiang Liu, Fang Dong, Xueyi Liu, Peimei Sun, Ran Zhao, Nan Onco Targets Ther Original Research Tumor cell vasculogenic mimicry (VM), a newly defined pattern of tumor blood supply, signifies the functional plasticity of aggressive cancer cells forming vascular networks. VM and cancer stem cells (CSCs) have been shown to be associated with tumor growth, local invasion, and distant metastasis. In our previous study, CSCs in triple-negative breast cancer were potential to participate in VM formation. In this study, breast CSCs were isolated from the triple-negative breast cancer cell line MDA-MB-231 by using mammosphere culture. Western blotting and reverse transcription polymerase chain reaction showed that mammosphere cells displayed an increased expression of AURKA protein kinase and stem cell marker c-myc and sox2. The VM formation by mammosphere cells was inhibited by AURKA knockdown or the addition of AURKA inhibitor MLN8237. In the meantime, MLN8237 induced the increased E-cadherin and decreased c-myc, sox2, and β-catenin expressions. The function of AURKA in VM formation was further confirmed using a xenograft-murine model. The results suggested that AURKA protein kinase is involved in VM formation of CSCs and may become a new treatment target in suppressing VM and metastasis of breast cancer. Dove Medical Press 2016-06-13 /pmc/articles/PMC4913546/ /pubmed/27366084 http://dx.doi.org/10.2147/OTT.S93015 Text en © 2016 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liu, Ying Sun, Baocun Liu, Tieju Zhao, Xiulan Wang, Xudong Li, Yanlei Meng, Jie Gu, Qiang Liu, Fang Dong, Xueyi Liu, Peimei Sun, Ran Zhao, Nan Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
title | Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
title_full | Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
title_fullStr | Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
title_full_unstemmed | Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
title_short | Function of AURKA protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
title_sort | function of aurka protein kinase in the formation of vasculogenic mimicry in triple-negative breast cancer stem cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913546/ https://www.ncbi.nlm.nih.gov/pubmed/27366084 http://dx.doi.org/10.2147/OTT.S93015 |
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