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Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs

BACKGROUND: Diagnosis of pancreatitis in dogs is complicated by extrapancreatic disorders that can alter the results of laboratory tests. Extrapancreatic disorders can also affect the diagnosis of exocrine pancreatic insufficiency (EPI). The effects of acute kidney injury (AKI) on pancreas‐specific...

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Autores principales: Hulsebosch, S.E., Palm, C.A., Segev, G., Cowgill, L.D., Kass, P.H., Marks, S.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913627/
https://www.ncbi.nlm.nih.gov/pubmed/26678019
http://dx.doi.org/10.1111/jvim.13806
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author Hulsebosch, S.E.
Palm, C.A.
Segev, G.
Cowgill, L.D.
Kass, P.H.
Marks, S.L.
author_facet Hulsebosch, S.E.
Palm, C.A.
Segev, G.
Cowgill, L.D.
Kass, P.H.
Marks, S.L.
author_sort Hulsebosch, S.E.
collection PubMed
description BACKGROUND: Diagnosis of pancreatitis in dogs is complicated by extrapancreatic disorders that can alter the results of laboratory tests. Extrapancreatic disorders can also affect the diagnosis of exocrine pancreatic insufficiency (EPI). The effects of acute kidney injury (AKI) on pancreas‐specific lipase activity (Spec cPL (®) Test), serum lipase activity and trypsin‐like immunoreactivity (TLI) in dogs have not been evaluated. HYPOTHESIS/OBJECTIVES: Serum Spec cPL, lipase activity, and TLI concentrations will increase secondary to decreased kidney function. ANIMALS: Five purpose‐bred dogs. METHODS: Experimental prospective study. Gentamicin was used to induce AKI in 5 purpose‐bred dogs. Serum samples were collected for measurement of creatinine, Spec cPL, lipase activity and TLI over 60 days, during both induction of, and recovery from, AKI. RESULTS: All dogs developed and recovered from AKI. Six of 52 (12%) serum Spec cPL concentrations were increased (2 in the equivocal zone and 4 consistent with pancreatitis) in 2 of 5 (40%) dogs. Two of 51 (4%) serum lipase activity values were increased in 2 of 5 dogs. Serum TLI was increased above the reference range in 17 of 50 (34%) samples in 3 of 5 dogs. For all biomarkers, there was no consistent correlation with increases in serum creatinine concentration. CONCLUSIONS AND CLINICAL IMPORTANCE: Decreased renal excretion during experimental AKI did not cause consistent and correlated increases in serum Spec cPL, lipase activity, or TLI in this cohort of dogs.
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spelling pubmed-49136272016-06-22 Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs Hulsebosch, S.E. Palm, C.A. Segev, G. Cowgill, L.D. Kass, P.H. Marks, S.L. J Vet Intern Med SMALL ANIMAL BACKGROUND: Diagnosis of pancreatitis in dogs is complicated by extrapancreatic disorders that can alter the results of laboratory tests. Extrapancreatic disorders can also affect the diagnosis of exocrine pancreatic insufficiency (EPI). The effects of acute kidney injury (AKI) on pancreas‐specific lipase activity (Spec cPL (®) Test), serum lipase activity and trypsin‐like immunoreactivity (TLI) in dogs have not been evaluated. HYPOTHESIS/OBJECTIVES: Serum Spec cPL, lipase activity, and TLI concentrations will increase secondary to decreased kidney function. ANIMALS: Five purpose‐bred dogs. METHODS: Experimental prospective study. Gentamicin was used to induce AKI in 5 purpose‐bred dogs. Serum samples were collected for measurement of creatinine, Spec cPL, lipase activity and TLI over 60 days, during both induction of, and recovery from, AKI. RESULTS: All dogs developed and recovered from AKI. Six of 52 (12%) serum Spec cPL concentrations were increased (2 in the equivocal zone and 4 consistent with pancreatitis) in 2 of 5 (40%) dogs. Two of 51 (4%) serum lipase activity values were increased in 2 of 5 dogs. Serum TLI was increased above the reference range in 17 of 50 (34%) samples in 3 of 5 dogs. For all biomarkers, there was no consistent correlation with increases in serum creatinine concentration. CONCLUSIONS AND CLINICAL IMPORTANCE: Decreased renal excretion during experimental AKI did not cause consistent and correlated increases in serum Spec cPL, lipase activity, or TLI in this cohort of dogs. John Wiley and Sons Inc. 2016 2015-12-17 /pmc/articles/PMC4913627/ /pubmed/26678019 http://dx.doi.org/10.1111/jvim.13806 Text en Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle SMALL ANIMAL
Hulsebosch, S.E.
Palm, C.A.
Segev, G.
Cowgill, L.D.
Kass, P.H.
Marks, S.L.
Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
title Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
title_full Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
title_fullStr Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
title_full_unstemmed Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
title_short Evaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
title_sort evaluation of canine pancreas‐specific lipase activity, lipase activity, and trypsin‐like immunoreactivity in an experimental model of acute kidney injury in dogs
topic SMALL ANIMAL
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913627/
https://www.ncbi.nlm.nih.gov/pubmed/26678019
http://dx.doi.org/10.1111/jvim.13806
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