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Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy

BACKGROUND: This study used 2 standardized methods to evaluate anti-PLA(2)R antibody in sera of Chinese patients with primary membranous nephropathy (PMN) and to determine whether immunological reactivity reflected by antibody titer correlates with kidney function parameters. MATERIAL/METHODS: Overa...

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Autores principales: Li, Xin, Wei, Dong, Zhou, Zhanmei, Wang, Baoguo, Xu, Ya, Pan, Jie, Yang, Chunli, Lu, Jie, Qiu, Yurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913829/
https://www.ncbi.nlm.nih.gov/pubmed/27179439
http://dx.doi.org/10.12659/MSM.896090
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author Li, Xin
Wei, Dong
Zhou, Zhanmei
Wang, Baoguo
Xu, Ya
Pan, Jie
Yang, Chunli
Lu, Jie
Qiu, Yurong
author_facet Li, Xin
Wei, Dong
Zhou, Zhanmei
Wang, Baoguo
Xu, Ya
Pan, Jie
Yang, Chunli
Lu, Jie
Qiu, Yurong
author_sort Li, Xin
collection PubMed
description BACKGROUND: This study used 2 standardized methods to evaluate anti-PLA(2)R antibody in sera of Chinese patients with primary membranous nephropathy (PMN) and to determine whether immunological reactivity reflected by antibody titer correlates with kidney function parameters. MATERIAL/METHODS: Overall, 82 subjects with biopsy-proven primary membranous nephropathy (PMN), 22 cases with secondary membranous nephropathy (SMN), 40 non-MN patients with established glomerulonephritis, and 20 healthy volunteers were recruited from the Division of Nephrology, Nanfang Hospital, China. Anti-PLA(2)R antibody in the serum of each patient was evaluated by both recombinant cell-based indirect immunofluorescence assay (RC-IFA) and enzyme-linked immunosorbent assay (ELISA). Kidney function was assessed by proteinuria for 24 hours, serum albumin, blood urea nitrogen (BUN), serum creatine, and serum cystatin C. We assessed the correlation between anti-PLA(2)R antibody levels and clinical parameters in the PMN patients. RESULTS: Fifty-three patients with PMN (64.6%) were positive for anti-PLA(2)R antibody. The level of antibody determined by RC-IFA ranged from 1: 10 to 1: 1000 and 0 to 1423 RU/ml by ELISA. The 2 anti-PLA(2)R test systems correlated very well with each other and reached an agreement of 95.7% for PMN patients. The level of antibody detected by ELISA in patients with PMN was also significantly correlated with proteinuria and nephritic-range proteinuria (>3.5 g/day). CONCLUSIONS: Anti-PLA(2)R antibody is sensitive and extremely specific for diagnosis of Chinese patients with primary membranous nephropathy. Concentration of autoantibody against PLA(2)R may be an ideal marker for monitoring the activity of immunological disease.
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spelling pubmed-49138292016-06-28 Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy Li, Xin Wei, Dong Zhou, Zhanmei Wang, Baoguo Xu, Ya Pan, Jie Yang, Chunli Lu, Jie Qiu, Yurong Med Sci Monit Clinical Research BACKGROUND: This study used 2 standardized methods to evaluate anti-PLA(2)R antibody in sera of Chinese patients with primary membranous nephropathy (PMN) and to determine whether immunological reactivity reflected by antibody titer correlates with kidney function parameters. MATERIAL/METHODS: Overall, 82 subjects with biopsy-proven primary membranous nephropathy (PMN), 22 cases with secondary membranous nephropathy (SMN), 40 non-MN patients with established glomerulonephritis, and 20 healthy volunteers were recruited from the Division of Nephrology, Nanfang Hospital, China. Anti-PLA(2)R antibody in the serum of each patient was evaluated by both recombinant cell-based indirect immunofluorescence assay (RC-IFA) and enzyme-linked immunosorbent assay (ELISA). Kidney function was assessed by proteinuria for 24 hours, serum albumin, blood urea nitrogen (BUN), serum creatine, and serum cystatin C. We assessed the correlation between anti-PLA(2)R antibody levels and clinical parameters in the PMN patients. RESULTS: Fifty-three patients with PMN (64.6%) were positive for anti-PLA(2)R antibody. The level of antibody determined by RC-IFA ranged from 1: 10 to 1: 1000 and 0 to 1423 RU/ml by ELISA. The 2 anti-PLA(2)R test systems correlated very well with each other and reached an agreement of 95.7% for PMN patients. The level of antibody detected by ELISA in patients with PMN was also significantly correlated with proteinuria and nephritic-range proteinuria (>3.5 g/day). CONCLUSIONS: Anti-PLA(2)R antibody is sensitive and extremely specific for diagnosis of Chinese patients with primary membranous nephropathy. Concentration of autoantibody against PLA(2)R may be an ideal marker for monitoring the activity of immunological disease. International Scientific Literature, Inc. 2016-05-15 /pmc/articles/PMC4913829/ /pubmed/27179439 http://dx.doi.org/10.12659/MSM.896090 Text en © Med Sci Monit, 2016 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
spellingShingle Clinical Research
Li, Xin
Wei, Dong
Zhou, Zhanmei
Wang, Baoguo
Xu, Ya
Pan, Jie
Yang, Chunli
Lu, Jie
Qiu, Yurong
Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy
title Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy
title_full Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy
title_fullStr Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy
title_full_unstemmed Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy
title_short Anti-PLA(2)R Antibodies in Chinese Patients with Membranous Nephropathy
title_sort anti-pla(2)r antibodies in chinese patients with membranous nephropathy
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913829/
https://www.ncbi.nlm.nih.gov/pubmed/27179439
http://dx.doi.org/10.12659/MSM.896090
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