Cargando…
MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor
MicroRNAs (miRs) have been demonstrated to play key roles in the development and progression of hepatocellular carcinoma (HCC). However, the regulatory mechanism of miR-503 in HCC has not been fully uncovered. In this study, we found that miR-503 was significantly downregulated in HCC tissues compar...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913972/ https://www.ncbi.nlm.nih.gov/pubmed/27366090 http://dx.doi.org/10.2147/OTT.S106351 |
_version_ | 1782438489223069696 |
---|---|
author | Xiao, Yao Tian, Qinggang He, Jiantai Huang, Ming Yang, Chao Gong, Liansheng |
author_facet | Xiao, Yao Tian, Qinggang He, Jiantai Huang, Ming Yang, Chao Gong, Liansheng |
author_sort | Xiao, Yao |
collection | PubMed |
description | MicroRNAs (miRs) have been demonstrated to play key roles in the development and progression of hepatocellular carcinoma (HCC). However, the regulatory mechanism of miR-503 in HCC has not been fully uncovered. In this study, we found that miR-503 was significantly downregulated in HCC tissues compared to nontumorous liver tissues. Moreover, lower miR-503 levels were associated with the malignant progression of HCC, and the expression of miR-503 was also decreased in several common HCC cell lines compared to normal human liver cell line THLE-3. Overexpression of miR-503 inhibited proliferation but induced apoptosis of LM3 and HepG2 cells. Bioinformatical analysis and luciferase reporter assay further identified insulin-like growth factor 1 receptor (IGF-1R) as a novel target of miR-503 in 293T cells. Moreover, overexpression of miR-503 led to a significant decrease in the protein levels of IGF-1R, while knockdown of miR-503 enhanced its protein levels in LM3 and HepG2 cells. Besides, overexpression of IGF-1R reversed the effects of miR-503-mediated HCC cell proliferation and apoptosis, indicating that IGF-1R acts as a downstream effector of miR-503 in HCC cells. Furthermore, IGF-1R was found to be significantly upregulated in HCC tissues compared to nontumorous liver tissues. In addition, the mRNA levels of IGF-1R were inversely correlated to the miR-503 levels in the HCC tissues. Thus, we demonstrate that miR-503 inhibits the proliferation and induces the apoptosis of HCC cells, partly at least, by directly targeting IGF-1R, and suggest that IGF-1R may serve as a promising target for the treatment of HCC. |
format | Online Article Text |
id | pubmed-4913972 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49139722016-06-30 MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor Xiao, Yao Tian, Qinggang He, Jiantai Huang, Ming Yang, Chao Gong, Liansheng Onco Targets Ther Original Research MicroRNAs (miRs) have been demonstrated to play key roles in the development and progression of hepatocellular carcinoma (HCC). However, the regulatory mechanism of miR-503 in HCC has not been fully uncovered. In this study, we found that miR-503 was significantly downregulated in HCC tissues compared to nontumorous liver tissues. Moreover, lower miR-503 levels were associated with the malignant progression of HCC, and the expression of miR-503 was also decreased in several common HCC cell lines compared to normal human liver cell line THLE-3. Overexpression of miR-503 inhibited proliferation but induced apoptosis of LM3 and HepG2 cells. Bioinformatical analysis and luciferase reporter assay further identified insulin-like growth factor 1 receptor (IGF-1R) as a novel target of miR-503 in 293T cells. Moreover, overexpression of miR-503 led to a significant decrease in the protein levels of IGF-1R, while knockdown of miR-503 enhanced its protein levels in LM3 and HepG2 cells. Besides, overexpression of IGF-1R reversed the effects of miR-503-mediated HCC cell proliferation and apoptosis, indicating that IGF-1R acts as a downstream effector of miR-503 in HCC cells. Furthermore, IGF-1R was found to be significantly upregulated in HCC tissues compared to nontumorous liver tissues. In addition, the mRNA levels of IGF-1R were inversely correlated to the miR-503 levels in the HCC tissues. Thus, we demonstrate that miR-503 inhibits the proliferation and induces the apoptosis of HCC cells, partly at least, by directly targeting IGF-1R, and suggest that IGF-1R may serve as a promising target for the treatment of HCC. Dove Medical Press 2016-06-15 /pmc/articles/PMC4913972/ /pubmed/27366090 http://dx.doi.org/10.2147/OTT.S106351 Text en © 2016 Xiao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Xiao, Yao Tian, Qinggang He, Jiantai Huang, Ming Yang, Chao Gong, Liansheng MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
title | MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
title_full | MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
title_fullStr | MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
title_full_unstemmed | MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
title_short | MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
title_sort | mir-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913972/ https://www.ncbi.nlm.nih.gov/pubmed/27366090 http://dx.doi.org/10.2147/OTT.S106351 |
work_keys_str_mv | AT xiaoyao mir503inhibitshepatocellularcarcinomacellgrowthviainhibitionofinsulinlikegrowthfactor1receptor AT tianqinggang mir503inhibitshepatocellularcarcinomacellgrowthviainhibitionofinsulinlikegrowthfactor1receptor AT hejiantai mir503inhibitshepatocellularcarcinomacellgrowthviainhibitionofinsulinlikegrowthfactor1receptor AT huangming mir503inhibitshepatocellularcarcinomacellgrowthviainhibitionofinsulinlikegrowthfactor1receptor AT yangchao mir503inhibitshepatocellularcarcinomacellgrowthviainhibitionofinsulinlikegrowthfactor1receptor AT gongliansheng mir503inhibitshepatocellularcarcinomacellgrowthviainhibitionofinsulinlikegrowthfactor1receptor |