miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1

miR-135a was downregulated in the majority of human primary gastric cancer (GC) tissues and GC cell lines. Kinesin family member C1 (KIFC1) was significantly upregulated in GC tissues and cell lines and promoted GC development and progression. We searched for miR-135a targets by using MiRanda, Targe...

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Autores principales: Zhang, Chuanlei, Chen, Xiaoqi, Chen, Xinju, Wang, Xinting, Ji, Aiying, Jiang, Lifeng, Sang, Feng, Li, Fucheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913988/
https://www.ncbi.nlm.nih.gov/pubmed/27366092
http://dx.doi.org/10.2147/OTT.S105736
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author Zhang, Chuanlei
Chen, Xiaoqi
Chen, Xinju
Wang, Xinting
Ji, Aiying
Jiang, Lifeng
Sang, Feng
Li, Fucheng
author_facet Zhang, Chuanlei
Chen, Xiaoqi
Chen, Xinju
Wang, Xinting
Ji, Aiying
Jiang, Lifeng
Sang, Feng
Li, Fucheng
author_sort Zhang, Chuanlei
collection PubMed
description miR-135a was downregulated in the majority of human primary gastric cancer (GC) tissues and GC cell lines. Kinesin family member C1 (KIFC1) was significantly upregulated in GC tissues and cell lines and promoted GC development and progression. We searched for miR-135a targets by using MiRanda, TargetScan, and PicTar tools, and found that KIFC1 was a potential target of miR-135a. Based on these findings, we speculated that miR-135a might target KIFC1 to inhibit GC growth. We determined the expression of miR-135a and KIFC1 by quantitative real-time polymerase chain reaction and Western blot assays, respectively, and found downregulation of miR-135a and upregulation of KIFC1 in GC tissues and cell lines. Cell proliferation and apoptosis assays showed that knockdown of KIFC1 inhibited proliferation and promoted apoptosis of GC cells, and miR-135a mimics had similar effects on GC cell proliferation and apoptosis. Furthermore, we verified that KIFC1 was a direct target of miR-135a, which confirmed our speculation that the functional effect of miR-135a on GC cells, at least, in part, depends on KIFC1. These findings suggest that miR-135a has an important role in the suppression of GC and presents a novel mechanism of miRNA-mediated KIFC1 expression in cancer cells.
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spelling pubmed-49139882016-06-30 miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1 Zhang, Chuanlei Chen, Xiaoqi Chen, Xinju Wang, Xinting Ji, Aiying Jiang, Lifeng Sang, Feng Li, Fucheng Onco Targets Ther Original Research miR-135a was downregulated in the majority of human primary gastric cancer (GC) tissues and GC cell lines. Kinesin family member C1 (KIFC1) was significantly upregulated in GC tissues and cell lines and promoted GC development and progression. We searched for miR-135a targets by using MiRanda, TargetScan, and PicTar tools, and found that KIFC1 was a potential target of miR-135a. Based on these findings, we speculated that miR-135a might target KIFC1 to inhibit GC growth. We determined the expression of miR-135a and KIFC1 by quantitative real-time polymerase chain reaction and Western blot assays, respectively, and found downregulation of miR-135a and upregulation of KIFC1 in GC tissues and cell lines. Cell proliferation and apoptosis assays showed that knockdown of KIFC1 inhibited proliferation and promoted apoptosis of GC cells, and miR-135a mimics had similar effects on GC cell proliferation and apoptosis. Furthermore, we verified that KIFC1 was a direct target of miR-135a, which confirmed our speculation that the functional effect of miR-135a on GC cells, at least, in part, depends on KIFC1. These findings suggest that miR-135a has an important role in the suppression of GC and presents a novel mechanism of miRNA-mediated KIFC1 expression in cancer cells. Dove Medical Press 2016-06-15 /pmc/articles/PMC4913988/ /pubmed/27366092 http://dx.doi.org/10.2147/OTT.S105736 Text en © 2016 Zhang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhang, Chuanlei
Chen, Xiaoqi
Chen, Xinju
Wang, Xinting
Ji, Aiying
Jiang, Lifeng
Sang, Feng
Li, Fucheng
miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1
title miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1
title_full miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1
title_fullStr miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1
title_full_unstemmed miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1
title_short miR-135a acts as a tumor suppressor in gastric cancer in part by targeting KIFC1
title_sort mir-135a acts as a tumor suppressor in gastric cancer in part by targeting kifc1
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913988/
https://www.ncbi.nlm.nih.gov/pubmed/27366092
http://dx.doi.org/10.2147/OTT.S105736
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